改良线栓法大鼠脑缺血再灌注损伤模型的建立  

Modified Establishment of Wistar Rats Ischemia-Reperfusion Injury Model

作　　者：陈晓娟[1] 肖宗宇[2] 潘琪[3] 王莉[4] 侯倩[1] 雷延成 吴世政[1] 

机构地区：[1]青海省人民医院,青海西宁810000 [2]青海大学附属医院 [3]海南医学院附属医院 [4]北京市普仁医院

出　　处：《中国医学创新》2015年第3期24-27,共4页Medical Innovation of China

基　　金：青海省科技计划项目(2012-Z-722)

摘　　要：目的：通过改良线栓法建立稳定的Wistar大鼠大脑中动脉脑缺血/再灌注模型。方法：60只雄性Wistar大鼠,参考Longa法并适当改进建立大鼠大脑中动脉脑缺血/再灌注模型30只,对照组30只。实验组结扎右侧颈总动脉近心端,夹闭颈总动脉远心端,颈总动脉剪一小口,从颈总动脉经颈内动脉插入线栓,缺血1 h后,在麻醉状态下完全拔出线栓,并结扎颈内动脉远心端。对照组结扎右侧颈内动脉近心端及远心端,但不插入线栓。术后取脑组织行TTC染色,并进行神经功能评定。结果：经改良线栓法制作脑缺血再灌注损伤模型,成功率达82.75%,平均操作时间约（20.4±5.3）min,模型大鼠右侧眼裂变小,行走时向右侧转圈,模型大鼠神经功能评分均在3-4分,经TTC染色梗死侧大脑半球颜色苍白,脑梗死区范围一致,梗死体积约（34.62±5.32）%;对照组无任何临床症状,无神经功能缺失表现,经TTC染色无梗死灶。结论：通过改良,成功建立了Wistar大鼠大脑中动脉脑缺血/再灌注模型。Objective：To establish a simple, reliable model of middle cerebral artery occlusion-reperfusion model in wistar rats.Method：On the basis of the method of Longa, 60 male wistar rats were randomly divided into two groups, 30 rats in each group. In the experimental group, the proximal part of the right common carotid artery（CCA） was ligated, the distal part of the CCA was clipped, then a small cut was made in CCA, thread was put into the internal carotid artery（ICA） through the cut of CCA to block the blood flow in the right of middle cerebral artery. One hour later, the thread was draw out under the condition of anesthetization, then the distal part of right CCA was ligated. In the control group, the proximal and distal part of the right common carotid artery（CCA） was ligated without putting thread into ICA. After operation, brain tissue was taken out to undergo TTC staining, and neurological deficit sign scores were performed.Result：The successful rate of modified MCAO/R model was 82.75%, the average time of operation was（20.4±5.3） min. In the experimental group, the right eye fission was smaller, the left extremities were paralyzed, and animals turn right while walking. The neurological deficit sign score was 3-4, the brain tissue supplied by right middle cerebral artery was pale under TTC staining, the areas of infarction was stable and the average area of infarction was about（34.62±5.32）%. There was no any neurological deficit in control group, and no positive results under TTC staining.Conclusion：Modified model of wistar rat ischemia-reperfusion injury has been established.

关 键 词：线栓法 大鼠 脑缺血 再灌注 

分 类 号：R-332[医药卫生]