白藜芦醇预处理对氧糖剥夺/再复氧损伤大鼠皮质神经干细胞增殖的影响  

Effect of resveratrol pretreatment on proliferation of cortical neural stem cells after oxygen-glucose deprivation/reoxygenation injury in rats

作　　者：成薇[1] 沈长波[1] 王莉[1] 余萍萍[1] 杨琴[1] 

机构地区：[1]重庆医科大学附属第一医院神经内科,重庆400016

出　　处：《中国药理学通报》2015年第1期113-118,共6页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金面上项目(No 81071119);国家神经病学临床重点专科建设项目(卫办;医政函[2012]649号]2012年)

摘　　要：目的研究白藜芦醇预处理对体外氧糖剥夺/再复氧损伤大鼠皮质神经干细胞增殖的影响。方法采用悬浮培养法分离纯化新生SD大鼠大脑皮质神经干细胞。第3代贴壁培养神经干细胞氧糖剥夺150 min后,复氧培养24 h。实验分为正常组、对照组、乙醇组和不同浓度白藜芦醇预处理组。免疫荧光法鉴定细胞,CCK-8法检测细胞活力,流式细胞周期及Brd U法检测细胞增殖。结果悬浮及贴壁培养细胞均高表达巢蛋白(nestin)。与对照组和乙醇组相比,不同浓度白藜芦醇预处理组(1、5、20μmol·L-1)均能明显增强细胞活力,促进细胞增殖,其中以5μmol·L-1白藜芦醇组作用最强(P<0.05)。结论白藜芦醇预处理能减轻氧糖剥夺/再复氧对神经干细胞的损伤,并促进其增殖。Aim To study the proliferative effect of resveratrol pretreatment on oxygen-glucose deprivation/reoxygenation （ OGD/R ） injury of rat cortical neural stem cells （ NSCs ） in vitro. Methods Isolation and purification of NSCs in neonatal Sprague-Dawley（ SD） rats were conducted by suspended cultivation. The third passage NSCs of adherent culture was cultured under oxygen and glucose deprivation for 150 min and reoxygenation for 24 h. The experimental subjects were divided into normal, control, ethanol and resveratrol pretreatment groups. Immunofluorescence was used to identify NSCs. Cell viability was detected with CCK-8 assay. Flow cytometry cell cycle and BrdU assay were used to measure cell proliferation. Results Cells both in suspended and adherent cultivation highly expressed neuroepithelial stem cell protein （ nestin ） . Compared with the control group, NSCs viabilities and prolifera-tion in resveratrol groups （1, 5, 20 μmol·L-1 ） were significantly heightened, and highest in the 5 μmol · L-1 resveratrol group （ P〈0. 05 ） . Conclusion Res-veratrol pretreatment can reduce injury and promote proliferation of NSCs after oxygen-glucose deprivation /reoxygenation.

关 键 词：白藜芦醇 不同浓度 预处理 神经干细胞 氧糖剥夺 再复氧损伤 细胞增殖 

分 类 号：R-332[医药卫生] R284.1