Bcl-2介导的芹菜素抗心肌细胞缺氧/复氧损伤作用  

Role of Bcl-2 signal pathway in apigenin preconditioning against cardiomyocytes anoxia/reoxygenation injury

作　　者：周敏[1] 游嘉振 何欢[2] 刘丹[2] 廖章萍[2] 汤蕾[2] 尹东[1] 何明[2] 

机构地区：[1]南昌大学第二附属医院江西省分子医学重点实验室,江西南昌330006 [2]南昌大学药学院江西省基础药理学重点实验室,江西南昌330006

出　　处：《中国药理学通报》2015年第1期122-127,共6页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 81160402)

摘　　要：目的探讨芹菜素(apigenin,Api)在心肌细胞缺氧/复氧损伤中的作用,并阐明Api对心肌损伤的保护作用是否主要由Bcl-2介导。方法培养H9c2心肌样细胞;随机分为正常对照(Cont)组、缺氧/复氧(Anoxia/Reoxygenation,A/R)组、Api预处理组、Api+ABT-737组。MTT法检测细胞存活率;Western blot法检测Bcl-2表达;比色法检测培养液LDH活性、细胞SOD及GSH-Px活性、MDA含量;流式细胞仪检测心肌细胞ROS含量、线粒体膜电位及细胞凋亡。结果 Api预处理25 h后,心肌细胞Bcl-2表达呈剂量依赖性上调(P<0.01);细胞存活率升高,培养液LDH活性降低,细胞SOD、GSH-Px活性升高,MDA含量与ROS生成减少,线粒体膜电位更为稳定,细胞凋亡减少(P<0.01);Bcl-2抑制剂ABT-737则可取消Api的上述心肌保护作用。结论Api抗心肌A/R损伤作用涉及Bcl-2信号通路,至少部分依赖于其对Bcl-2表达水平的上调。Aim To investigate the relationship be-tween the cardioprotection of apigenin （ Api ） from an-oxia/reoxygenation （ A/R） injury and Bcl-2 pathway. Methods H9 c2 cardiomyocytes were cultured and di-vided into normal control group, A/R group, Api pre-treatment group （ Api ） , Api ＋ Bcl-2 inhibitor group （ Api ＋ ABT-737 ） . Expression of Bcl-2 was deter-mined by Western blot,and cell viability was measured by MTT method. LDH, SOD, GSH-Px, MDA activity were determined by chromometry. ROS generation, mi-tochondrial membrane potential and apoptosis were de-termined by flow cytometry. Results 25h after apige-nin precondition,the expression of Bcl-2 was upregulat-ed in cardiomyocytes （ P 〈0. 01 ） . In the group pre-treated with 40 μmol · L-1 apigenin before A/R, the activity of LDH in culture medium decreased; the ac-tivity of intracellular SOD, GSH-Px increased; the content of MDA and ROS generation decreased; cell viability increased; mitochondrial membrane potential could be more stable and cell apoptosis decreased （ P〈0. 01 ） . However, all these protective effects were attenuated significantly in the group pretreated with apigenin and Bcl-2 inhibitor ABT-737 . Conclusion The effect of apigenin against A/R injury in cardiomyo-cytes involves Bcl-2 pathway, and at least partly de-pends on its effect on upregulating the expression of Bcl-2 .

关 键 词：芹菜素 BCL-2 缺氧/复氧损伤 心肌细胞保护 细胞凋亡 线粒体 

分 类 号：R-332[医药卫生] R284.1