杜仲提取物中五个成分血浆蛋白结合率的测定  

Determination of plasma protein binding rate of five components in Eucommia ulmoides extract

作　　者：曹旭[1] 谢玉敏[1] 朱迪[1] 陈鹏程[1] 巩仔鹏[1] 王爱民[2] 

机构地区：[1]贵阳医学院药学院贵州省药物制剂重点实验室,贵州贵阳550004 [2]贵阳医学院民族药与中药开发应用教育部工程研究中心,贵州贵阳550004

出　　处：《中国药理学通报》2015年第1期131-135,共5页Chinese Pharmacological Bulletin

基　　金：贵州省科技重大专项项目(黔科合重大专项字[2012]6009号);贵州省中药现代化研究开发专项(黔科合中药字[2013]5062号;黔科合重G字[2013]4001号);贵州省高等学校创新能力提升计划(黔教合协同创新字[2013]04)

摘　　要：目的测定杜仲药材中5个成分的血浆蛋白结合率。方法采用平衡透析法测定血浆蛋白结合率,生物样本用甲醇沉淀蛋白进行处理,以葛根素为内标,利用超高效液相色谱-串联质谱仪测定血浆和缓冲溶液中的5个成分浓度。结果在所研究浓度范围内,京尼平苷酸、原儿茶酸、绿原酸、松脂醇二葡萄糖苷和松脂醇单葡萄糖苷的平均蛋白结合率分别为(25.77±2.68)%、(57.54±3.79)%、(53.91±3.00)%、(24.15±4.92)%、(49.78±3.61)%。结论京尼平苷酸和松脂醇二葡萄糖苷的蛋白结合率较低,原儿茶酸、绿原酸和松脂醇单葡萄糖苷则与大鼠血浆蛋白有中等强度的结合。Aim To determine the plasma protein binding rate of five components of Eucommia ulmoides extract. Methods The equilibrium dialysis method was used to study the plasma protein binding rate. The plasma samples were extracted by protein precipitation with methanol. With the use of puerarin as the internal standard, UPLC-MS/MS was carried out to determine the concentration of the five compounds in and out of the dialysis membrane. Results The average plasma protein binding rates of five compounds on the area of the concentration which was determinate were as fol-lows, respectively： geniposidic acid was （ 25. 77 ± 2. 68 ）%, protocatechuic acid was （ 57. 54± 3. 79）%, chlorogenic acid was （53. 91 ± 3. 00）%, pinoresinol diglucoside was （24. 15 ± 4. 92）%, and pinoresinol singleglucoside was （49. 78 ± 3. 61）%. Conclusions The results show that the binding percentage of geniposidic acid and pinoresinol diglucoside is relatively low, but the binding rate of the others with rat plasma protein is moderate.

关 键 词：杜仲 京尼平苷酸 原儿茶酸 绿原酸 松脂醇二葡萄糖苷 松脂醇单葡萄糖苷 平衡透析法 液质联用 

分 类 号：R-332[医药卫生] R282.71