大鼠慢性前列腺炎模型中TRPA1及相关炎症因子的表达  

Expressions of transient receptor potential A1 and related inflammatory factors in the rat model of prostatic inflammation

作　　者：黄宝星[1] 曹万里[1] 黄欣[1] 戴军[1] 宿恒川[1] 成康[1] 孙福康[1] 

机构地区：[1]上海交通大学医学院附属瑞金医院泌尿外科,上海200025

出　　处：《中华男科学杂志》2015年第1期23-30,共8页National Journal of Andrology

基　　金：云南白药集团股份有限公司

摘　　要：目的：探讨模拟慢性前列腺炎/慢性盆腔疼痛综合征（CP/CPPS）动物模型疼痛产生的分子机制。方法：取SD大鼠36只,随机分为实验组和对照组,每组18只。实验组向前列腺双腹侧叶注射50μl 3%无菌λ-角叉菜胶制作大鼠无菌性前列腺炎症性疼痛模型,对照组注射等量无菌生理盐水。两组分别于造模后1周、2周和4周3个时间节点,每个节点6只大鼠,解剖获取大鼠前列腺组织、L6~S1段背根神经节（DRG）及脊髓,采用免疫组化联合Western印迹法检测神经生长因子（NGF）、瞬时受体电位通道蛋白A1（TRPA1）及降钙素基因相关肽（CGRP）在不同组织内的表达情况。结果：慢性前列腺炎症性疼痛大鼠前列腺组织中NGF、CGRP、TRPA1蛋白的表达均高于对照组（P〈0.05）,且随造模时间的延长各蛋白表达逐渐减弱,组间比较差异有统计学意义（P〈0.05）。在大鼠L6~S1段DRG及脊髓内,造模后1周,实验组NGF、CGRP及TRPA1表达与对照组比较差异无统计学意义（P〉0.05）,造模后2周及4周,各蛋白表达呈持续高水平状态,与造模后1周大鼠比较差异无统计意义（P〉0.05）,但各实验组大鼠与对照组蛋白表达比较差异均有统计学意义（P〈0.05）,两时间点上两组蛋白表达比较差异无统计学意义（P〉0.05）。结论：前列腺内注射λ-角叉菜胶方法制作的大鼠前列腺炎症性疼痛模型,可在分子水平模拟CP/CPPS的产生机制;TRPA1在CP/CPPS患者疼痛产生上可能发挥中继通道的作用。Objective： To explore the molecular mechanism of pain associated with chronic prostatitis and chronic pelvic pain syndrome（ CP / CPPS） in the rat model of prostatic inflammation. Methods： Thirty-six male SD rats were equally randomized to an experimental and a control group,the former injected with 50 μl of 3% λ-carrageenan into the ventral prostate to make the model of non-bacterial prostatic inflammation,while the latter with the same volume of sterile saline solution. At 1,2 and 4 weeks after modeling,the prostate,L6- S1 dorsal root ganglion（ DRG） and spinal cord were harvested for examination of the expressions of the nerve growth factor（ NGF）,transient receptor potential ankyrin 1（ TRPA1）,and calcitonin-gene-related peptide（ CGRP） by immunohistochemistry and Western blot. Results： The expressions of NGF,TRPA1 and CGRP in the prostatic tissue were all significantly increased in the experimental group as compared with the control（ P 〈 0. 05）,with a gradual decrease with the prolonging of time（ P 〈0. 05）. In the L6- S1 DRG and spinal cord,the expressions of NGF,TRPA1 and CGRP exhibited no significant differences between the experimental and control groups at 1 week after modeling（ P 〉 0. 05） and kept at high levels in the experimental group at 2 and 4weeks,though not significantly different from those at 1 week（ P 〉 0. 05）. Statistically significant differences were observed in the expressions of the three proteins in the experimental rats among different time points（ P 〈 0. 05）,but not between the two groups at any time point（ P 〉 0. 05）. Conclusion： The molecular mechanism of CP / CPPS can be evaluated in the rat model of prostatic inflammation established by injecting λ-carrageenan into the prostate. TRPA1 may play an important role in connecting the upstream and downstream pathways of CP / CPPS-associated pain.

关 键 词：前列腺炎 疼痛 瞬时受体电位通道蛋白A1 神经生长因子 降钙素基因相关肽 

分 类 号：R-332[医药卫生] R697.33