TGFα/EGFR通路相关蛋白在B6-Co小鼠眼睑中的表达  

Expression of TGFα/EGFR signal pathway-associated protein in B6-Co mice embryonic eyelids

作　　者：缪进[1] 李瑶[1] 刘春[1] 宋鸿雁[1] 邵义祥[1] 

机构地区：[1]南通大学实验动物中心,南通大学比较医学研究所,江苏省南通市226001

出　　处：《眼科新进展》2015年第1期28-31,共4页Recent Advances in Ophthalmology

基　　金：江苏省高校自然科学基金资助(编号:13KJB180019)~~

摘　　要：目的研究转化生长因子α(transforming growth factorα,TGFα)/表皮生长因子受体(epidermal growth factor receptor,EGFR)通路相关蛋白在B6-Co胎鼠眼睑中的表达情况,探讨B6-Co小鼠眼睑发育异常的原因。方法取胚胎16.5 d、17.5 d、18.5 d的B6和B6-Co胎鼠头部,做冰冻切片,通过免疫荧光法检测各时间点TGFα在眼睑部位的原位表达情况;提取蛋白,采用Western blot法检测TGFα表达及EGFR、细胞外调节蛋白激酶(extracellular regulated protein kinase,ERK)磷酸化蛋白表达情况。结果免疫荧光检测结果示,TGF氉α主要表达于眼睑间质区,3个时间点B6-Co胎鼠眼睑中TGFα表达的荧光强度均高于同一时间点在B6胎鼠眼睑中的表达。Western blot检测结果示,3个时间点B6-Co胎鼠眼睑中TGFα、p-EGFR的表达与B6胎鼠相比,差异均无统计学意义(均为P>0.05)。p-ERK在胚胎16.5 d和17.5 d时,B6-Co胎鼠眼睑中的表达量分别为3.79±0.32和3.77±0.16,均显著高于在相应时间点B6胎鼠眼睑中的表达量1.48±0.11和1.45±0.07,差异均有统计学意义(均为P<0.05)。结论 TGFα/EGFR通路的下游因子ERK可能是导致B6-Co小鼠眼睑异常发育的重要因子。Objective To investigate the expression of transforming growth factor α （TGFα）/ epidermal growth factor receptor （EGFR） signal pathway-associated protein in embryonic eyelids, and explore the abnormal eyelid developmental mechanism in B6-Co mice. Methods El6.5 days,E17.5 days,E18.5 days 136 and BS-Co embryonic heads were collected, and frozen sections were made by cryotomy. Immunofluorescence staining for TGFα was applied in the fetuses eyelids, and Western blot was used to analyze the expression of TGFα,p-EGFR and p-extraceilular regulated protein kinases （p-ERK） both in 136 and B6-Co mice. Results TGFα mainly expressed in the mesenchyme and fluorescence intensity in all B6-Co mice were higher than those in 136 mice at E15.5 days,E17.5 days,E18.5 days. There was no statistical difference in TGFα and p-EGFR expression between B6-Co and B5 mice detected by Western blot （ all P 〉 0.05 ）. The p-ERK expression at E15.5 days,E17.5 days in B6-Co mice embryonic eye- lids were 3.79 ±0.32 and 3.77 ±0.16,respectively,which in B6 mice were 1.48 ±0.11 and 1.12 ±0.09 ,respectively, there were statistical differences （ all P 〈 0.05 ）. Conclusion ERK in TGFα/EGFR signal pathway may be one of the most significant down- stream factors leading abnormal eyelid development in B6-Co mice.

关 键 词：B6-Co小鼠 眼睑 转化生长因子Α 表皮生长因子受体 细胞外调节蛋白激酶 

分 类 号：R735.2[医药卫生—肿瘤]