ASE快速溶剂萃取与测定青海十六种高寒植物中多糖含量  

Accelerated Solvent Extraction and Determination of Polysaccharides in 16 Alpine Plants from Qinghai

作　　者：谭亮[1] 冀恬[1] 耿丹丹[1] 胡风祖[1] 

机构地区：[1]中国科学院西北高原生物研究所,西宁810008

出　　处：《天然产物研究与开发》2014年第12期1992-1999,共8页Natural Product Research and Development

基　　金：中国科学院知识创新工程重要方向项目(KSCX2-EW-J-26);农产品安全检测公共技术服务平台项目(2012-T-Y19)

摘　　要：本研究采用快速溶剂萃取法（ASE）提取青海十六种高寒植物中的多糖,并建立了苯酚-硫酸法测定其多糖含量。使用ASE350通过在线净化过程加入乙醚除去样品中的脂类物质,加入75%乙醇在线除去游离单糖、低聚糖和糖醛酸,同时对样品进行脱色。继续加入蒸馏水用ASE在线提取出样品中的多糖。离心除去由淀粉溶胀糊化形成的胶体物质,上清液加入Sevag试剂除去蛋白质,试液依次加入5%苯酚溶液和浓硫酸显色。以蒸馏水做空白对照,在490 nm处测定吸光度,同时采用水提醇沉法进行多糖换算因子的测定。结果表明,各样品多糖在0.01-0.10 mg范围内具有良好的线性关系（r=0.9996）,平均加样回收率为97.26%,RSD为0.53%（n=9）。该方法简便、准确、稳定性和重现性好,适用于测定植物样品中多糖的含量,可为青海高寒植物的质量控制提供依据。In this study,an accelerated solvent extraction（ ASE） method was developed for extraction of polysaccharides in 16 alpine plants from Qinghai. A phenol-vitriolic colorimetry method was set up for determination of polysaccharides contents. The lipids were removed using ASE350 with diethyl ether through on-line purification. Monosaccharides,oligosaccharides and uronic acid were on-line removed with 75% aqueous ethanol,meanwhile the samples were bleached with it. The plant polysaccharides were continuously on-line extracted by distilled water through ASE. Colloidal substances that were formed by swelling of gelatinized starch were removed by centrifugation,proteins in the supernatant were removed with Sevag reagent. The 5% phenol solution and concentrated sulfuric acid were sequencely added in the test solutions for chromogenic reaction. The absorbance was monitored at 490 nm using distilled water as blank. Polysaccharide conversion factors were measured. The results indicated that the linear response range of polysaccharides in samples was0. 01 - 0. 10 mg（ r = 0. 9996）. The average recovery（ n = 9） was 97. 26% and RSD was 0. 53%. The assay demonstrated that the method was simple,accurate,stable and reproducible. It was suitable for the determination of polysaccharides in plant samples. In addition,it can be used to assist the quality evaluation of alpine plants from Qinghai.

关 键 词：快速溶剂萃取 在线除杂质和提取 苯酚-硫酸法 青海高寒植物 多糖 含量测定 

分 类 号：R284.2[医药卫生—中药学]