柴达木栽培和野生枸杞子中东莨菪内酯含量的测定  

Determination of Scopoletin in the Wild and Cultivated Fructus Lycii from Qaidam Basin

作　　者：曹静亚[1,2] 谭亮[1,2] 迟晓峰[1,2] 胡风祖[1,2] 

机构地区：[1]中国科学院藏药研究重点实验室 [2]中国科学院西北高原生物研究所,西宁810008

出　　处：《天然产物研究与开发》2014年第12期2004-2007,共4页Natural Product Research and Development

基　　金：中国科学院知识创新工程重要方向项目(KSCX2-EW-J-26)

摘　　要：建立测定枸杞子中东莨菪内酯的HPLC方法,并进行方法学考察。利用所建立的方法对柴达木栽培和野生枸杞子的东莨菪内酯含量进行测定。以Diamonsil C18柱为分析柱,甲醇-0.05%磷酸溶液（35∶65）为流动相,流速：1 m L/min,柱温：30℃,检测波长：344 nm;研究发现,枸杞子中东莨菪内酯在0.026-1.040μg（R2=0.9996）范围内成良好的线性关系;平均加样回收率（n=6）为96.1%,RSD=2.0%。该方法准确、可靠、重现性好,适用于枸杞子中东莨菪内酯的含量测定。测定结果表明,柴达木栽培枸杞子具有很整齐的品质优良性,而野生枸杞子品质差异很大,适宜于选择性的开发。A high performance liquid chromatography（ HPLC） method was developed and validated to determine the contents of scopoletin in the wild and cultivated Fructus Lycii from Qaidam Basin. The component was separated on a Diamonsil C18 columnusing methanol-0. 05% phosphoric acid buffer（ 35∶ 65） as the mobile phases. The flow rate was 1 m L /min. The column temperature was set at 30 ℃ and the detection wavelength was set at 344 nm. It was found that the linearity range of scopoletin was from 0. 026-1. 040 μg（ R2= 0. 9996）. The average recoveries（ n = 6） were 96. 1%,RSD =2. 0%. The method was accurate,reliable and reproduciblefor the determination ofscopoletin in Fructus Lycii. The result showed that the quality of cultivated Fructus Lyciifrom Qaidam Basin was neatly excellent. However,the wild samples had the significant different qualities,which was suitable for the selective exploitation.

关 键 词：高效液相色谱 东莨菪内酯 柴达木 枸杞子 

分 类 号：O657.7[理学—分析化学]