中华鳖源奇异变形杆菌的分离鉴定与致病性研究  

Isolation and Identification of Pathogen Proteus mirabilis from Diseased Chinese Soft-shelled Turtle Pelodiscus sinensis

作　　者：林亚歌 叶键[2] 石婷婷[1] 宋阳[1] 王玉凤[1] 章晓栋[1] 

机构地区：[1]绍兴文理学院生命科学学院,浙江绍兴312000 [2]杭州市水产技术推广总站,浙江杭州310000

出　　处：《水产科学》2014年第12期800-803,共4页Fisheries Science

基　　金：绍兴市重点科技计划项目(No:2011A22008)

摘　　要：从患典型鳃腺炎病中华鳖肝脏和腹水处进行细菌接种,分离获得一株细菌。综合梅里埃细菌生化鉴定系统和16SrRNA序列进化分析,判断所分离菌株为奇异变形杆菌(SX37)。动物回归试验显示,该分离菌株能够引起幼鳖发病死亡,并且能够从试验幼鳖体内再次分离到相同的病原菌。但临床症状与病理解剖结果与自然发病症状不一致,显示该菌株不是中华鳖鳃腺炎病病原,而是继发病原。分离株对小鼠的半数致死量为107.83,显示该菌株具有较强的致病性。One bacterium was isolated from liver and ascites of diseased Chinese soft-shelled turtle （Pelodiscus sinensis）,outbreaked in a large-scale breeding farm in Shangyu, and identified as Proteus mirabilis, named SX37 through API ID32E by 16S rRNA sequencing, and biochemical reactions. Healthy softshelled turtle was experimentally infected with the bacterial suspension, and then the same bacterium was recovered with different clinic sydromes. Isolate SX43 demonstrated virulence potential in mouse models, with the median lethal dose （LD50） of 1 × 107.83cfu/mL in mice.

关 键 词：中华鳖 奇异变形杆菌 16S RRNA 

分 类 号：S947.1[农业科学—水产养殖]