HPLC测定蒙药嘎日迪五味丸中莽草酸和没食子酸的含量  

Determination of Shikimic Acid and Gallic Acid in Mongolian Medicine Garidi-5 Pills by HPLC

作　　者：肖田梅[1] 席海山[1] 白梅荣[1] 陈玉花[1] 

机构地区：[1]内蒙古民族大学化学化工学院,内蒙古通辽028043

出　　处：《内蒙古民族大学学报（自然科学版）》2014年第5期585-587,共3页Journal of Inner Mongolia Minzu University：Natural Sciences

基　　金：国家民委资助项目(12NMZ007)

摘　　要：目的：建立同时测定蒙药嘎日迪五味丸中莽草酸和没食子酸的HPLC方法，为该蒙药复方制剂的质量控制提供一种简便、快速、准确的方法.方法：采用岛津LC-10ATVP高效液相色谱仪，色谱柱为ODS2（250mm×4.6mm，5μm）（大连中汇达科学仪器公司）；SPD-10AVP检测器；柱温25℃.结果：莽草酸在0.1～0.5μg，没食子酸在0.05～0.25μg范围内，莽草酸和没食子酸的浓度与相应的峰面积呈良好的线性关系.莽草酸的回归方程为Y=1.0×107 X-2.1×104, r=0.9999；没食子酸的回归方程为Y=9.0×108 X＋3.8×105，r=0.9997，莽草酸、没食子酸的平均回收率分别为101.0%,RSD=1.60%；101.6%，RSD=1.75%.结论：本法简便、快捷、重复性好，结果准确可靠，可为研究蒙药嘎日迪五味丸的质量标准方法的建立提供可靠依据.Objective：To establish the simultaneous determination method of shikimic acid and gallic acid in Garidi-5 pills by HPLC, and to provide a simple, rapid and accurate method for the quality control of Mongolian compound prep-arations. Methods： Shimadzu LC-10ATVP HPLC was used, the column was ODS2 （250mm × 4.6mm,5μm, Dalian Delta Scientific Instrument Company）;detector was SPD-10AVP;column temperature was 25℃. Results：the gshikim-ic acid in the range of 0.1-0.5μg, the gallic acid in the range of 0.05-0.25μg, the concentration and the correspond-ing peak area showed a good linear relationship, the regression equation of shikimic acid was Y=1.0 × 107 X-2.1 × 104,r=0.9999;the regression equation of gallic acid was Y=9.0×108 X＋3.8×105, r=0.9997;The average recovery of shikimic acid and gallic acid were 101.0%（RSD=1.60%）and 101.6%（RSD=1.75%.） respectively. Conclusion：The method is easy, rapid and reproducible, the result is accurate and reliable, which can provide a good basis for establish-ing the quality standard control for Garidi-5 pills.

关 键 词：高效液相谱法 蒙药嘎日迪五味丸 莽草酸 没食子酸 

分 类 号：R284.2[医药卫生—中药学]