FISH检测c-MYC/IgH基因重排在淋巴组织增生性疾病中的意义  

Significance of c-MYC/IGH translocation detected by fluorescence in situ hybridization(FISH)in lymphoprolifetative disease

作　　者：王明华[1] 蒙秋萍 罗志飞[1] 陈星言[1] 解娜[1] 张弦[1] 黄幼生[1] 翁阳[1] 

机构地区：[1]海南医学院病理教研室/附属医院病理科,海南海口571101

出　　处：《海南医学院学报》2014年第12期1601-1603,1606,共4页Journal of Hainan Medical University

基　　金：海南省自然科学基金项目(812187);海南省教育厅高校科研资助项目(Hjkj2012-30)~~

摘　　要：目的:探讨c-MYC/IgH基因重排在淋巴组织增生性疾病中的意义及其临床应用价值。方法:收集本院病理科的淋巴组织增生性病变10例,应用EBER原位杂交检测EBV感染情况,荧光原位杂交(FISH)检测淋巴组织增生性疾病c-MYC/IgH基因重排,PCR检测IgH/IgK或TCRγ基因重排,分析FISH技术在淋巴组织增生性疾病中的诊断和鉴别诊断意义。结果:10例淋巴组织增生性病变中仅1例EBV感染,所有病例均未检测到c-MYC/IgH基因重排,部分病例检测到c-MYC/IgH或TCRγ基因单克隆性重排。结论:通过FISH检测c-MYC/IgH基因重排在淋巴组织增生性疾病中的诊断价值尚需进一步探讨。Objective： To explore the significance of c-MYC/IGH translocation detected by fluorescence in situ hybrid- ization （FISH） in Lymphoprolifetative Disease. Methods： All LPD tissues investigated in this study were obtained from pa- tients （n= 10） treated in the Affiliated Hospital of Hainan Medical College. CD20 ,CD3 ,CD5 ,CD10 ,CD21 ,Bcl-2 ,Bcl-6 ,Mum-1 and Ki-67 protein by immunohistochemical array were detected in all cases. At the same time, all cases were detected by EBER in situ hybridization, c-MYC/IGH by FISH and IgH/IgK or TCRγ by PCR assay. Clinicopathological data were collected and the relationship between AID expression and clinicopathological feature was analyzed. Results： Of the 10 cases, only 1 LPD showed EBER positive, c-MYC/IgH gene rearrange was not observed and TCRγ gene rearrangement/ c-MYC/IGH mono- clonal sex was detected in some cases. Conclusion： The significance of c-MYC/IGH translocation detected by FISH in LPD need be further discussed and studied.

关 键 词：荧光原位杂交(FISH) c-MYC/IgH基因重排 淋巴组织增生性疾病 

分 类 号：R733.4[医药卫生—肿瘤]