miRNA-143通过抑制KRAS降低前列腺癌细胞的增殖  

miRNA-143 decreases human prostate cancer cell proliferation through suppression of KRAS

作　　者：牛晓兵[1] 许斌[2] 张翔翔[2] 王增军[2] 

机构地区：[1]南京医科大学附属淮安第一医院泌尿外科,江苏省223300 [2]南京医科大学第一附属医院泌尿外科

出　　处：《中华临床医师杂志（电子版）》2014年第21期12-14,共3页Chinese Journal of Clinicians(Electronic Edition)

基　　金：国家自然科学基金(81270685)

摘　　要：目的探讨miRNA-143对前列腺癌细胞增殖的影响及其作用机制。方法通过RT-PCR和Western blot方法检测miRNA-143及其靶基因KRAS在前列腺癌组织中的表达;用MTT方法和集落形成实验研究miRNA-143对激素非依赖性前列腺癌DU145细胞增殖的影响。结果 miRNA-143及其靶基因KRAS在前列腺癌组织中的表达呈负相关;miRNA-143高表达对DU145细胞的增殖具有抑制作用;DU145细胞中miRNA-143高表达抑制KRAS-ERK信号通路的激活及Cyclin D1的表达。结论 miRNA-143通过RAS/MAPK信号通路抑制前列腺癌的增殖,为激素非依赖性前列腺癌治疗提供了分子生物学基础。Objective To verify its effect and mechanisms on prostate cancer cell proliferation. Methods miR.NA-143 and its target gene KRAS expression was measured by real-time PCR and western blotting and effects of miRNA-143 in cell proliferation and migration were evaluated by MTT and colony formation. Results An inverse correlation between miRNA-143 and KRAS expression in prostate cancer tissues. Over-expression of miRNA-143 in DU145 cells suppressed their proliferation by targeting RAS/MAPK pathway. Conclusion miRNA-143 plays an important role in prostate cancer cell proliferation by suppressing KRAS and subsequent inactivation of MAPK pathway, which provide a new approach for HRPC treatment.

关 键 词：前列腺肿瘤 miRNA-143 KRAS HRPC MAPK 

分 类 号：R737.25[医药卫生—肿瘤]