响应面法优化黑土豆色素提取工艺研究  

Optimization of extraction technique of pigment from Black Potato by response surface methodology

作　　者：任雪峰[1,2] 王永生[1,2] 丁秀丽[1] 安红钢[1,2] 吴冬青[1,2] 

机构地区：[1]河西学院化学化工学院,甘肃张掖734000 [2]甘肃省高校河西走廊特色资源利用省级重点实验室,甘肃张掖734000

出　　处：《广西植物》2014年第6期859-864,共6页Guihaia

基　　金：甘肃省教育厅研究生导师项目(1210-01)

摘　　要：以黑土豆为原料提取色素,在乙醇浓度、回流温度、回流时间和料液比四个单因素实验基础上,通过响应面法优化提取条件,利用Box-Benhnken设计模型,研究四个自变量之间的影响。结果表明,得到最佳提取工艺为回流温度47℃,回流时间47.5 min,料液比1∶90,乙醇浓度42.5%。在此条件下,理论上测得黑土豆中色素的吸光度值为0.413,实验测得黑土豆中色素的吸光度值为0.416,理论值与实验值的相对误差为0.726%。该提取工艺简便、具有较高的重现性和可行性。The optimum conditions for extraction pigment from Black Potato were determined by using response sur-face method on the basis of ethanol concentration,reflux temperature,reflux time and ratio of solid to liquid four sin-gle factor tests.The results showed that the optimal extraction condition was as follows：ethanol concentration 42.5%,reflux temperature 47 ℃,reflux time 47.5 min,and ratio of solid to liquid 1∶90.Under these conditions,the measured absorbance of pigment was 0.413,compared with the predictive value of 0.416,the relative error was 0.726%.The result also indicated it was feasible to adopt response surface methodology to optimize the extraction condition.

关 键 词：响应面法 黑土豆 色素 提取工艺 

分 类 号：Q946[生物学—植物学] TS201.1[轻工技术与工程—食品科学]