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作 者:陈娟[1] 罗毅[1] 王丽芝[1] 秦娟[2] 高微微[1]
机构地区:[1]中国医学科学院北京协和医学院药用植物研究所,北京100193 [2]北京市丰台区疾病预防控制中心,北京100071
出 处:《菌物学报》2015年第1期150-158,共9页Mycosystema
基 金:Supported by the National Natural Science Foundation of China(30973882);Ministry of Health Public Research Foundation(201002001)
摘 要:报道了曲霉属的3个中国新记录种:Aspergillus dimorphicus,A.fumigatiaffinis以及A.westerdijkiae。比较了其与相近种的形态及分子序列β‐tubulin基因的差异,并采用高效液相色谱‐质谱联用技术对菌株产赭曲霉毒素A(OTA)的能力进行了测定。检测结果表明Aspergillus westerdijkiae CGMCC3.15268在大米培养基上能够产生OTA,单位菌丝的产毒量为17.26μg/kg;而A.fumigatiaffinis CGMCC3.15275以及A.dimorphicus CGMCC3.17045未检出OTA。Three species of Aspergillus namely A. dimorphicus, A. fumigatiaffinis and A. westerdijkiae were newly recorded in China. The morphological characteristics and β‐tubulin gene difference were compared with allied species. The ochratoxin A(OTA) produc‐ing ability was analyzed by using liquid chromatography/mass spectrometry/mass spectrometry(LC/MS/MS). Aspergillus west‐erdijkiae CGMCC3.15268 could produce OTA at the level of 17.26μg/kg on rice medium, while A. fumigatiaffinis CGMCC3.15275 and A. dimorphicus CGMCC3.17045 were OTA negative.
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