胃癌BRCA1基因甲基化与BRCA1mRNA表达的关系研究  

Relationship Between BRCA1 Gene Methylation Status and BRCA1 mRNA Expression Level in Gastric Cancer

作　　者：孙欣欣[1] 王红兵[2] 徐海洋[2] 

机构地区：[1]徐州医学院研究生学院,江苏徐州221002 [2]徐州医学院附属医院肿瘤内科,江苏徐州221002

出　　处：《肿瘤基础与临床》2014年第6期463-466,共4页journal of basic and clinical oncology

摘　　要：目的探讨乳腺癌易感基因1(BRCA1)启动子Cp G岛甲基化与BRCA1 mRNA表达的相关性及其意义。方法采用甲基化特异性PCR技术检测37例胃癌组织和对应癌旁组织及6例正常胃组织中BRCA1基因启动子Cp G岛甲基化状态;采用逆转录PCR技术检测37例胃癌组织和对应癌旁组织及6例正常胃组织中BRCA1 mRNA表达水平。结果胃癌组织中BRCA1基因启动子Cp G岛总甲基化率为48.6%(18/37),显著高于癌旁组织[5.4%(2/37)]和正常胃组织[0.0%(0/6)],差异有统计学意义(χ2=17.541、5.021,P均<0.05)。胃癌组织中BRCA1 mRNA阳性表达率为48.6%(18/37),显著低于癌旁组织[94.6%(35/37)]和正常胃组织[100.0%(6/6)],差异有统计学意义(χ2=19.215、5.520,P均<0.05)。BRCA1基因启动子Cp G岛甲基化与BRCA1 mRNA表达成负相关(r=-0.515,P<0.05)。结论 BRCA1基因启动子Cp G岛甲基化可能是导致BRCA1 mRNA失表达的主要原因之一。Objective To investigate the relationship between the methylation of breast cancer susceptibility gene 1（BRCA1）promoter CpG island and the expression level of BRCA1 mRNA in gastric cancer and the signifi-cance. Methods Methylation-specific PCR was used to detect the methylation of BRCA1 gene promoter CpG island in the tumor tissues and paired adjacent cancerous tissues from 37 patients gastric cancer and the 6 normal gastric tissues. reverse transcription PCR was performed to detect the expression of BRCA1 mRNA in the tumor tissues and paired ad-jacent cancerous tissues from 37 patients gastric cancer and the 6 normal gastric tissues. Results The methylation rate of the BRCA1 gene promoter CpG island in the gastric cancer tissues[48. 6%（18/37）]was higher than those in the paired adjacent tissues[5. 4%（2/37）]and the normal gastric tissues[0. 0%（0/6）]（χ2 =17. 541,5. 021;P〈0. 05）. The expression positive rate of the BRCA1 mRNA in the gastric cancer tissues[48. 6%（18/37）]was higher than those in the paired adjacent tissues[94. 6%（35/37）]and the normal gastric tissues[100. 0%（6/6）]（χ2 =19. 215,5. 520;P 〈0. 05 ）. The methylation of BRCA1 gene promoter CpG island has a negative correlation with BRCA1 mRNA（r= -0. 515,P〈0. 05）. Conclusion Methylation of BRCA1 gene promoter CpG island may be one of the main reasons for the loss of BRCA1 mRNA expression.

关 键 词：胃癌 BRCA1基因 甲基化 

分 类 号：R735.2[医药卫生—肿瘤] R730.23[医药卫生—临床医学]