人参炔醇对人胰腺癌细胞PANC-1增殖的抑制作用  

Inhibitory effects of panaxynol on proliferation of human pancreatic cancer cell PANC-1

作　　者：吴荧荧 谢晓东[1] 张礼荣[1] 王颖[1] 黄文斯 陈芳芳[1] 朱海涛[1] 王冬青[1] 

机构地区：[1]江苏大学附属医院影像科,212000

出　　处：《江苏医药》2015年第1期7-10,共4页Jiangsu Medical Journal

基　　金：江苏省自然科学基金(BK2011487);镇江市社会发展项目(SH2013026)

摘　　要：目的探讨人参炔醇(PNN)对人胰腺癌细胞PANC-1增殖的抑制作用。方法将人胰腺癌细胞PANC-1分为3个PNN浓度(1、9、27μmol/L)组和对照(C)组,MTT检测各组细胞增殖情况,流式细胞术检测各组细胞周期变化,Western blot检测各组细胞Ki67蛋白表达。结果 PNN组细胞抑制率和G1期细胞比例均呈浓度依赖性增加,而Ki67蛋白表达量呈浓度依赖性递减(P<0.01)。结论 PNN可能通过抑制细胞分裂和Ki67表达对人胰腺癌细胞PANC-1增殖发挥抑制作用。Objective To investigate the inhibitory effects of panaxynol（PNN）on the proliferation of human pancreatic cancer cell PANC-1.Methods PANC-1cells were divided into three PNN groups（treated with PNN 1,9and 27μmol/L）and group C（control）.The proliferation of PANC-1cells,changes of cell cycle and Ki67 expression were detected by MTT,flow cytometry and Western blot,respectively.Results Among PNN groups,the inhibition rate and cell proportion in G1 phase were increased and Ki67 expression was reduced in PNN concentration-dependent manner（P〈0.01）.Conclusion PNN has inhibitory effects on the proliferation of human pancreatic cancer cell PANC-1through inhibiting cell division and down-regulating Ki67 expression.

关 键 词：人参炔醇 人胰腺癌细胞PANC-1 

分 类 号：R735[医药卫生—肿瘤]