两种脾虚证模型对小鼠骨骼肌形态和功能的影响  

Effect of Two Pi Deficiency Syndrome Models on the Configuration and Function of the Skeletal Muscle in Mice

作　　者：张全旺[1] 李光月 任艳萍[1] 高云芳[1] 

机构地区：[1]西北大学生命科学学院生物科学与技术系,西安710069 [2]西安交通大学医学院第一附属医院科技部,西安710061

出　　处：《中国中西医结合杂志》2015年第1期71-75,共5页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助项目(No.31270455);2011年度高等学校博士学科点专项科研基金项目(No.20116101110013);陕西省国际科技合作与交流计划项目(No.2013KW26-01)

摘　　要：目的探讨脾虚证与骨骼肌收缩功能的关系。方法将36只ICR小鼠按体重配对原则随机分成3组,即对照组、劳倦过度组及大黄组,每组12只。其中劳倦过度组及大黄组应用大黄泻下法和力竭游泳加剥夺睡眠的方法制备两种脾虚证动物模型;采用m-ATPase酶组织化学方法测定3组小鼠趾长伸肌(extensor digitorum longus,EDL)和比目鱼肌(soleus,SOL)m-ATPase酶活性的变化,计算Ⅰ型和Ⅱ型肌纤维的比例;Power Lab系统测定EDL和SOL等长收缩和强直收缩的最大张力。结果劳倦过度组及大黄组小鼠的体重、体温与一般健康状况均明显下降;脾脏、胸腺脏器指数亦低于对照组;等长收缩最大张力和强直收缩最大张力明显下降;EDL和SOL的肌纤维横截面积减小,细胞排列松散;EDL中Ⅰ型肌纤维比例增加,Ⅱ型肌纤维比例降低,SOL中Ⅰ型和Ⅱ型肌纤维比例无变化。结论劳倦过度组及大黄组小鼠的EDL和SOL均出现明显萎缩,劳倦过度组SOL中Ⅰ型肌纤维萎缩更加明显。Objective To observe the relation between Pi deficiency syndrome（ PDS） and the configuration and functions of extensor digitorum longus（ EDL） and soleus（ SOL）. Methods Totally 36 ICR mice were randomly divided into3 groups according to weight matching principle,the control group,the exhausted group,and the rhubarb group,12 in each group. Two PDS models were established by either purgation with rhubarb diarrhea（ as Group A） or exhausted swimming plus sleep deprivation（ as Group B）. The cross sectional area（ CSA） of type Ⅰ and Ⅱ fibers of extensor digitorum longus（ EDL） and soleus（ SOL）,relative proportions of type Ⅰ and Ⅱ fibers were measured by m-ATPase histochemical method.The isotonic contraction and the maximum tetanus contraction of EDL and SOL were detected by Power Lab system. Results Compared with the control group,the body weight,body temperature,and the general health condition of PDS model rats obviously decreased; the spleen index and the thymus index were also lower; the maximal isotonic contraction and the maximum tetanus contraction obviously decreased; the cross section areas of EDL and SOL were reduced with loosely arranged cells. In EDL,the proportion of type Ⅰ fibers was added and the proportion of type Ⅱ fibers was lowered. In SOL,there was no change in the proportion of type Ⅰ and type Ⅱ fibers. Conclusions EDL and SOL were obviously atrophied in the two PDS model mice. The type Ⅰ fibers of SOL was more significantly atrophied in Group B.

关 键 词：脾虚证 趾长伸肌 比目鱼肌 等长收缩 强直收缩 

分 类 号：R-332[医药卫生] R228