睾酮对青春期大鼠病理性攻击行为及前额叶皮质PSD-95和GAP-43表达的影响  

Effect of testosterone on pathological aggression behavior and expression of postsynaptics density-95and growth associated protein-43in prefrontal cortex in puberty rats

作　　者：吕小娟[1] 张艳[1] 胡华[1] 陈竹[1] 秦光成[2] 桂蓓[2] 陈力学[2] 

机构地区：[1]重庆医科大学附属第一医院心理卫生中心,重庆400016 [2]重庆医科大学附属第一医院实验研究中心,重庆400016

出　　处：《第二军医大学学报》2015年第1期39-43,共5页Academic Journal of Second Military Medical University

基　　金：重庆市自然科学基金(CSTC;2011jjA10104);重庆医科大学国家自然科学基金预研项目(NSFYY201111)~~

摘　　要：目的探讨去势后睾酮水平对青春期大鼠病理性攻击行为和前额叶皮质突触可塑性的影响。方法将30只出生后21d的雄性SD大鼠随机分为去势组、模型组和对照组。去势组和模型组大鼠采用国际通用应激方法建立病理性攻击模型,持续到青春期。采用居住-入侵实验检测青春期大鼠攻击行为,ELISA法检测睾酮水平,蛋白质印迹分析检测前额叶皮质突触后致密物质95(PSD-95)和生长相关蛋白43(GAP-43)表达水平。结果居住-入侵实验结果显示,去势组攻击潜伏期显著低于模型组和对照组(P<0.01,P<0.01),屈服后继续攻击次数高于模型组和对照组(P<0.01,P<0.01)。ELISA检测结果显示,去势组睾酮水平显著低于模型组和对照组(P<0.01,P<0.01),且睾酮水平与攻击各指标呈中-高度负相关(P<0.01)。蛋白质印迹结果显示,去势组前额叶皮质PSD-95和GAP-43表达水平均低于模型组和对照组(P<0.01,P<0.01)。结论低水平血清睾酮对青春期大鼠前额叶皮质结构可塑性有损伤,可能与其病理性攻击行为相关。Objective To explore the effect of serum testosterone level on the pathologically aggressive behavior and the synaptic plasticity in the prefrontal cortex of puberty rats after gonadectomy. Methods Thirty male rats, 21 days old, were randomly divided into 3 groups： gonadectomized group, model group and control group. The gonadectomized and model groups were given a series of standard stress from 21 days old to puberty to induce animal model of pathological aggressive behavior. Resident-intruder experiment was performed to observe the variation of aggressive behaviors of animals. Enzyme-linked immunosorbent assay was used to examine the serum testosterone level. Western blotting analysis was used to determine the expression of postsynaptics density-95 （PSD-95） and growth associated protein-43 （GAP-43） in the prefrontal cortex. Results Resident-intruder experiment showed that the latency to the first attack in the gonadectomized group decreased significantly （P〈0.01 ,P〈0.01） and the attack times after yield increased significantly compared with those in the other two groups （P〈0.01,P〈0.01）. The serum testosterone in the gonadectomized group was significantly decreased compared with the other two groups as shown by ELISA results （P〈0.01, P〈0.01）. In addition, the aggressive-related behavior indices had a moderate to high negative correlation with the testosterone level （P〈0.01）. Western blotting analysis showed that prefrontal cortex expression of PSD-95 and GAP-43 in the gonadectomized group was significantly lower than those in the other two groups （P〈0. 01, P〈0. 01）. Conclusion Low serum testosterone level can cause damage to the neuroplasticity of prefrontal cortex in puberty rats, which might be related to the pathologically aggressive behavior.

关 键 词：睾酮 病理性攻击 PSD-95 GAP-43 前额叶皮质 

分 类 号：R749[医药卫生—神经病学与精神病学]