磁性隐形阿霉素脂质体的制备及其对SKOV-3细胞影响初步研究  

The preparation of doxorubicin-loaded Fe_3O_4 magnetic nanoparticles modified with PEG and the effect of SKOV-3

作　　者：陈万瑛 陈钰[2] 杨蕊[3] 李向东 任预应 王亚鹏 唐秋莎[3] 陈道桢[1,2] 

机构地区：[1]新疆阿合奇县人民医院检验科,新疆克州843500 [2]南京医科大学附属无锡妇幼保健院中心实验室,江苏无锡214002 [3]东南大学医学院病理与生理学系,江苏南京210009

出　　处：《中国实验诊断学》2014年第6期880-883,共4页Chinese Journal of Laboratory Diagnosis

基　　金：新疆维吾尔自治区自然科学基金资助项目(201318101-13)

摘　　要：目的制备磁性隐形阿霉素脂质体,对其理化性质及其热疗后对SKOV-3细胞的影响进行评价。方法使用pH梯度法制备磁性隐形阿霉素脂质体,通过透射电镜观察其形态,通过紫外分光光度计测试其包封率,差示扫描量热仪测定其相变温度,MTT及流式细胞仪测定凋亡率。结果不同脂质体粒径在80-150nm间,包封率达到76%,相变温度为42.3℃,MTT及流式的结果均显示热疗对SKOV-3细胞的杀伤作用显著高于对照组。结论磁性隐形阿霉素脂质体包封率较高,粒径较小,对SKOV-3具有显著影响。Objective Aim To prepare the doxorubicin-loaded Fe3 O4 magnetic nanoparticles modified with PEG,to study the physicochemical properties of the nanoparticles and the effects on SKOV-3 cells after hyperthermia.Methods Using the pH gradient method to prepare the liposomes,the characteristics were observed with TEM,UV spectro-photometer,DSC,and the apoptosis rate was determined by MTT and flow cytometry.Results The particle size of li-posomes was during 80-150 nm,the encapsulation ratio detected by UV spectrophotometer spherical was 76%.The DSC showed the liposomes changed their phase at 42.3℃.The result of MTT and flow cytometry showed cytotoxic effect of hyperthermia on SKOV-3 cells was significantly better than that of control group.Conclusion These doxoru-bicin liposomes with high encapsulation ratio and small particle size might be high efficiency on SKOV-3 cells.

关 键 词：热疗 阿霉素 脂质体 卵巢癌 

分 类 号：R737.31[医药卫生—肿瘤]