重组人粒细胞集落刺激因子对脑出血后caspase-3表达影响的实验研究  

Experimental Study of rhG-CSF on the expression of Caspase-3 in Treatment of Intracerebral Hemorrhage

作　　者：贾丽君[1] 刘群[2] 王伟[3] 

机构地区：[1]解放军第208医院神经内科,吉林长春130000 [2]吉林大学第一医院神经内科,吉林长春130021 [3]吉林大学再生医学科学研究所,吉林长春130021

出　　处：《中国实验诊断学》2014年第6期888-889,共2页Chinese Journal of Laboratory Diagnosis

基　　金：吉林省自然科学基金项目(201115132)

摘　　要：目的应用重组人粒细胞集落刺激因子(rhG-CSF)对脑出血(ICH)大鼠神经功能损伤的影响进行评估。方法随机将实验动物分为对照组、治疗组和假手术组。治疗组于术后1h始腹部皮下注射rhG-CSF,在相应时间点大鼠行神经功能评分(NSS)后处死,用RT-PCR方法观察各组各时程病灶周围脑组织caspase-3的动态表达。结果治疗组神经功能评分明显低于对照组,rhG-CSF治疗减少了caspase-3的表达,二者相比有差异显著(P<0.05)。结论 rhG-CSF治疗对神经细胞具有保护作用。Objective To investigate the effection of rhG-CSF on Caspase-3 in Treatment of intracerebral hemor-rhage rats.Methods Experimental rats were assigned randomly the ICH group,the treating group and the sham opera-tion group,The treating group was administered rhG-CSF by intraperitoneal injection post 1 h.Before killing the rats neurological severity score was evaluated on each time point,then the expression of Caspase-3 were measured using RT-PCR.Results the neurological severity scores and expression of Caspase-3 in the treating group reduced significantly than those in the ICH group.Conclusion the rhG-CSF treatment have neuroprotective effects.

关 键 词：重组人粒细胞集落刺激因子 脑出血 CASPASE-3 

分 类 号：R743.3[医药卫生—神经病学与精神病学]