培美曲塞联合β-榄香烯对宫颈癌 HeLa 细胞增殖和凋亡的影响  

β-elemene combined with pemetrexed inhibits the proliferation and increases the apoptosis of HeLa cells

作　　者：白杨[1] 李录[1] 马力天 张毅[3] 毛立挺 马瑞[4] 任秦有[3] 胡月[1] 邢劲松[1] 郑瑾[3] 

机构地区：[1]第四军医大学2009级医学生,西安710032 [2]解放军68222部队卫生队,陇西748000 [3]第四军医大学唐都医院中医科暨中西医结合肿瘤科,西安710032 [4]第四军医大学西京医院放疗科,西安710032

出　　处：《医学研究生学报》2015年第1期7-10,共4页Journal of Medical Postgraduates

基　　金：国家自然科学基金(81072973);陕西省中医管理局中医药科研课题(lc70)

摘　　要：目的培美曲塞和β-榄香烯均可抑制肿瘤细胞的生长。文中探讨培美曲塞联合β-榄香烯对宫颈癌HeLa细胞增殖和凋亡的影响。方法体外培养宫颈癌HeLa细胞,将培美曲塞(浓度为38、76、152、228、304μg/m L)单独作用于HeLa细胞后24、48 h用MTT比色法检测细胞的增殖情况,实验分β-榄香烯组(125μg/m L)、培美曲塞组(76μg/m L)、联合用药组(培美曲塞76μg/m L,β-榄香烯125μg/m L)及空白对照组(0μg/m L),24 h后用MTT法检测细胞的增殖情况,用流式细胞术检测24 h细胞的凋亡率。结果 MTT法显示,培美曲塞浓度在38、76、152、228、304μg/m L梯度范围内对HeLa细胞有抑制作用,随浓度增加,抑制率增强。38、76、152、228、304μg/m L的培美曲塞作用24后,增殖抑制率分别为(7.24±3.78)%、(7.94±4.37)%、(11.10±2.86)%、(15.88±3.38)%、(16.52±2.54)%;其中38、76、152、228μg/m L培美曲塞抑制率两两比较的差异有统计学意义(P<0.05)。联合用药组24 h抑制率[(49.95±5.76)%]高于β-榄香烯组[(24.36±5.59)%]和培美曲塞组[(10.69±1.37)%],差异有统计学意义(P<0.01)。结论培美曲塞与β-榄香烯联合应用可抑制HeLa细胞的增殖,协同促进HeLa细胞的凋亡。Objective Pemetrexed and β-elemene can inhibit the growth of tumor cells .This study was to investigate the effect of pemetrexed combined with β-elemene on the proliferation and apoptosis of cervical cancer HeLa cells. Methods Cervical cancer HeLa cells were treated with pemetrexed at the concentrations of 38, 76, 152, 228, and 304μg/mL, and at 24 and 48 hours of treatment subjected to MTT for detection of their proliferation .The experiment included four groups , with the cells treated with β-elemene （ 125μg/mL） , pemetrexed （ 76 μg/mL ） , β-elemene （ 125 μg/mL ） ＋pemetrexed （76μg/mL）, and nothing （blank control） for 24 hours, followed by determination of their proliferation and apoptosis by MTT and flow cytometry, respectively. Results Pemetrexed at 38, 76, 152 and 228μg/mL inhibited the proliferation of the HeLa cells in a concentration-dependent manner, with the inhibition rates of （7.24 ±3.78）, （7.94 ±4.37）, （11.10 ±2.86） and （15.88 ± 3.38）%at 24 hours, and （16.69 ±0.95）, （22.54 ±1.53）, （24.48 ±0.92） and （25.54 ±3.61）%at 48 hours, both with statis-tically significant differences between any two groups （P〈0.05）.Significant differences were also found in the proliferation rate of the same concentration of pemetrexed at the two time points （P〈0.05）.The combination of pemetrexed and β-elemene showed an inhibi-tion rate of （49.95 ±5.76）%at 24 hours, remarkably higher than （24.36 ±5.59）%in theβ-elemene group and （10.69 ±1.37）%in the pemetrexed group （P〈0.01）. Conclusion Pemetrexed combined with β-elemene can significantly inhibit the proliferation and synergistically accelerate the apoptosis of HeLa cells .

关 键 词：Β-榄香烯 培美曲塞 HELA细胞 增殖 凋亡 

分 类 号：R737.33[医药卫生—肿瘤]