MicroRNA-21对食管鳞状上皮癌细胞TE-1生物学特性的影响  

Effect of microRNA-21 on biological characteristics of esophageal squamous carcinoma cells TE-1

作　　者：黄珊[1] 陈鑫[2] 马红兵[1] 惠蓓娜[2] 张丽[2] 马海琳[2] 张晓智[2] 

机构地区：[1]西安交通大学医学院第二附属医院肿瘤放疗科,陕西西安710004 [2]西安交通大学医学院第一附属医院肿瘤放疗科,陕西西安710061

出　　处：《现代肿瘤医学》2015年第3期294-298,共5页Journal of Modern Oncology

基　　金：国家自然科学基金资助项目(No.30972962)

摘　　要：目的:食管鳞状上皮癌TE-1细胞中microRNA-21(miR-21)高表达,本研究探讨miR-21对TE-1细胞生物学特性的影响。方法:转染miRZip-21慢病毒,持久抑制TE-1细胞中高表达的miR-21,将由此获得的稳定细胞系命名为anti-miR-21细胞系,实时定量PCR方法验证,以TE-1细胞作为对照。细胞增殖实验检测anti-miR-21细胞增殖能力,Transwell侵袭实验观察细胞侵袭能力,Transwell迁移实验及划痕实验观察细胞迁移能力,克隆形成实验及细胞毒性实验分别观察放射线及药物敏感性变化。结果:Anti-miR-21细胞的相对增殖率较对照组TE-1细胞低,差异具有统计学意义(P<0.05)。Transwell侵袭实验及迁移实验显示穿过小室的anti-miR-21细胞较TE-1细胞分别减少51.97%及64.31%,差异具有统计学意义(P<0.05)。划痕实验显示anti-miR-21细胞较TE-1细胞的迁移率降低,差异具有统计学意义(P<0.01)。克隆形成实验分析显示anti-miR-21细胞的D0值(2.73Gy vs 3.60Gy)和Dq值(1.25Gy vs 2.75Gy)均低于对照组,差异具有统计学意义(P<0.05)。细胞毒性实验显示anti-miR-21细胞在顺铂及氟尿嘧啶各个梯度浓度下细胞存活率均降低,差异具有统计学意义(P<0.05)。结论:MiR-21影响食管鳞状上皮癌TE-1细胞的生物学特性。抑制miR-21表达后,降低了TE-1细胞的增殖、侵袭及迁移能力,同时增加了其对放射及化学药物的敏感性。MiR-21可能成为食管鳞癌治疗的潜在靶点。Objective： MicroRNA - 21 （ miR - 21 ） is over - expressed in esophageal squamous carcinoma cells TE - 1. This study investigated the effect of miR -21 on the biological characteristics of TE - 1 cells. Methods：By lenti- viral transduction with miRZip -21, the microRNA -21 expression in TE - 1 cells was stably down -regulated, which was renamed as ＂anti - miR -21 cells＂. TE - 1 cells was used as control. The proliferation change of anti - miR -21 cells was tested by cell proliferation assay. Transwell invasion assay was used to observe the invasive ability. Transwell migration assay and sear assay were used to observe the migration change. The sensitivities of radiation and chemo- therapeutic drugs were tested by clonogenic assay and cytotoxicity assay respectively. Results ：The relative cell prolif- eration rate of anti - miR - 21 cells was lower compared with the control group TE - 1 cells （ P 〈 0.05 ）. Transwell invasion and migration assay showed that the cells which through the small chamber in anti - miR - 21 group （P 〈 0.05 ）, decreased 51.97% and 64.31% respectively, compared with TE - 1 cells. Scar assay showed that the mob- ility of anti - miR - 21 decreased than that of TE - 1 cells （ P 〈 0.05 ）. Clonogenic assay showed that DO （2.73Gy vs 3.60Gy） and Dq （ 1.25Gy vs 2.75Gy） of the anti - miR - 21 cells were lower than those of control cells （ P 〈 0.05 ）. The sensitivities of cisplatin and fluorouracil of anti - miR - 21 cells were statistically significantly increased （P 〈 O. 05 ）. Conclusion： MiR -21 affects the biological characteristics of esophageal squamous carcinoma TE - 1 cells. Inhibition of miR - 21 reduces the proliferation, invasion and migration of TE - 1 cells, while increasing their sensitivi- ties to radiation and chemotherapeutic drugs. MiR -21 could be a potential target for the treatment of esophageal squamous cell carcinoma.

关 键 词：食管鳞癌 TE-1细胞 MICRORNA-21 生物学特性 

分 类 号：R73-36[医药卫生—肿瘤] R735.1[医药卫生—临床医学]