荧光铁载体与牛血清白蛋白作用的光谱研究  

Spectral Studies on the Interaction of Pyoverdine and Bovine Serum Albumin

作　　者：安鸣[1] 许彩虹[2] 孟晶岩[1] 杨斌盛[2] 

机构地区：[1]山西省农业科学院农产品加工所,山西太原030031 [2]山西大学分子科学研究所,山西太原030006

出　　处：《山西大学学报（自然科学版）》2015年第1期120-124,共5页Journal of Shanxi University(Natural Science Edition)

基　　金：山西省回归国留学基金(No.201013);山西省科技推广项目(201210702009);教育部博士点基金(20131401110011)

摘　　要：在pH=8.0、50mmol·L-1 Tris-HCl缓冲溶液及室温条件下,用荧光光谱、紫外可见吸收光谱及寿命荧光测定等方法研究了荧光铁载体Pyoverdine(Pvd)与牛血清白蛋白(BSA)的相互作用.研究表明,通过疏水作用Pvd与BSA结合形成1∶1的稳定复合物,其表观结合常数为log K=7.26±0.12,结合位点距色氨酸残基间的距离r为4.16nm.The interaction between fluorescent siderophore pyoverdine,Pvd and bovine serum albumin,BSA was studied in 50mmol·L^-1 Tris-HCl buffer solution,pH 8.0,at room temperature by using fluorescence and ultraviolet-visible absorption spectroscopy.The results show that the binding of Pvd with BSA is in a molar ratio of 1∶1and the conditional stability constant is log K=7.26±0.12.The binding process involves a positive entropy change.The acting force for the binding reaction between Pvd and HSA is mainly a hydrophobic interaction.The distance（r）between Pvd and tryptophan of BSA was determined as 4.16 nm based on Frster non-radiative energy transfer theory.

关 键 词：荧光铁载体 牛血清白蛋白 荧光 热力学 

分 类 号：O657.3[理学—分析化学]