上海市猪源沙门氏菌耐药性及脉冲场凝胶电泳分型研究  

Research on Drug-resistance and Genotyping of Salmonella from Pig in Shanghai by Pulsed-fi eld Gel Electrophoresis

作　　者：宁昆[1] 张维谊[1] 沈莉萍[1] 徐峰[1] 齐新永[1] 王建[1] 

机构地区：[1]上海市动物疫病预防控制中心,上海201103

出　　处：《中国动物检疫》2015年第2期78-81,共4页China Animal Health Inspection

基　　金：沪农科攻字(2011)第4-9号

摘　　要：为了解上海地区猪源沙门氏菌的耐药性及基因分型情况,本研究对2013年分离到的13株猪源沙门氏菌(10株为猪霍乱沙门氏菌,3株为鼠伤寒沙门氏菌)采用微量肉汤稀释法进行药物敏感性测定,应用脉冲场凝胶电泳(PFGE)进行基因分型。药物敏感试验结果表明,分离到的13株沙门氏菌对磺胺异恶唑、多西环素、氟苯尼考和阿莫西林的耐药率较高,对阿莫西林/棒酸、头孢曲松、丁胺卡那霉素和头孢噻呋敏感。脉冲场凝胶电泳分型结果显示,10株猪霍乱沙门氏菌中有8株为流行病学相关,是本次研究中猪霍乱沙门氏菌的优势基因型。To investigate the drug-resistance and genotype of Salmonella from pig in Shanghai, 13 Salmonella isolates （10 Salmonella choleraesuis, 3 Salmonella typhimurium） were tested for their drug-resistance using broth dilution method, and genotyped by pulsed-field gel electrophoresis （PFGE） . Drug-resistance test showed that the 13 Salmonella isolates were resistant to sulfisoxazole, doxycycline, florfenicol and amoxicillin, and sensitive to amoxieillin/clavulanic acid, ceftriaxone, amikacin and ceftiofur. Pulsed-field gel electrophoresis typing showed that 8 Salmonella choleraesuis were epidemiologically linked, and were the dominant genotype in our study.

关 键 词：上海 猪 沙门氏菌 耐药性 脉冲场凝胶电泳 

分 类 号：R378.22[医药卫生—病原生物学] S828[医药卫生—基础医学]