垂体腺瘤组织中NF-κB、MMP-9、MICA的表达变化及意义  

Expression and significance of NF-kappa B,MMP-9,MICA in pituitary adenoma tissues

作　　者：陈正[1] 常映薇 李珍珠[1] 孙其凯 王清波[1] 李泽福[1] 

机构地区：[1]滨州医学院附属医院,山东滨州256600 [2]滨州医学院烟台校区

出　　处：《山东医药》2015年第3期1-4,共4页Shandong Medical Journal

基　　金：国家自然科学基金资助项目(81171119)

摘　　要：目的观察垂体腺瘤组织中NF-κB、基质金属蛋白酶9(MMP-9)、MHC-Ⅰ类链分子相关抗原A(MICA)的表达变化,并探讨其意义。方法采用免疫组化法对垂体腺瘤组织和正常脑组织中的NF-κB蛋白、MMP-9蛋白及MICA蛋白进行检测;采用qRT-PCR法对垂体腺瘤组织及正常脑组织中的NF-κB mRNA、MMP-9 mRNA、MICA mRNA进行检测。结果垂体腺瘤与正常脑组织中NF-κB蛋白阳性表达率分别为100%(40/40)、30%(6/20),MMP-9蛋白阳性表达率分别为100%(40/40)、15%(3/20),MICA蛋白阳性表达率分别为100%(40/40)、0;两组比较,P均<0.01。垂体腺瘤与正常脑组织中NF-κB mRNA相对表达量分别为13.045±6.200、2.040±2.067,MMP-9 mRNA相对表达量分别为28.770±7.661、2.369±1.460,MICA mRNA相对表达量分别为231.817±41.046、5.062±3.849;两组比较,P均<0.05。NF-κB蛋白与MICA蛋白、MMP-9蛋白表达均呈正相关(r分别为0.368、0.345,P均<0.05)。结论 NF-κB、MMP-9及MICA在垂体腺瘤组织中高表达,NF-κB可能通过促进MMP-9及MICA表达而促进垂体腺瘤发生、发展。Objective The aim of this study was to examine the expression levels of NF-κB, MMP-9, and MICA and to explore its significance. Methods We examined the association between NF-κB, MMP-9, and MICA by immunohisto- chemical staining, Quantitative real-time PCR. Results The positive rate of NF-κB were 100% （40/40）, 30% （6/20） in pituitary adenomas and normal brain tissue. The positive rate of M MP-9 were 100% （40/40）, 15 % （3/20）in pituitary ade- nomas and normal brain tissue. The positive rate of MICA were 100% （40/40）, 0 in pituitary adenomas and normal brain tissues（P 〈0.05）. Relative to express the NF-κB mRNA were 13. 045 ±6.2, 2.04 ± 2. 067 in pituitary adenomas and normal brain tissues. Relative to express the MMP-9 mRNA were 28.77±7. 661, 2. 369 ± 1.46 in pituitary adenomas and normal brain tissues. Relative to express the MICA mRNA were 231. 817 ±41. 046, 5. 062 ±3. 849 in pituitary adenomas and normal hrain tissues（ P 〈0.05 ）. The expression levels of NF-κB, MMP-9, and MICA were positively related in pa- tients with pituitary adenomas （r=0.368,0.345,P〈0.05）. Conclusions NF-κB, MMP-9, and MICA are highly ex- pressed in pituitary adenomas tissues. High levels of NF-κB by promoting the expression of MMP-9 and MICA promote the development of pituitary adenomas.

关 键 词：垂体瘤 MHC-Ⅰ类链分子相关抗原A 基质金属蛋白酶9 核转录因子-ΚB 

分 类 号：R736.4[医药卫生—肿瘤]