利用污泥水解酸化液合成PHAs菌株的分离和鉴定  

Isolation and Identification of Bacterial Strain for Synthesizing Polyhydroxyalkanoates with Solution from Sludge

作　　者：马宗敏[1,2] 王慧[1] 洪思平 闫海[4] 

机构地区：[1]清华大学环境学院,北京100084 [2]河北省中医药研究院,河北石家庄050031 [3]扬州澄露环境工程有限公司,江苏扬州225000 [4]北京科技大学化学与生物工程学院,北京100083

出　　处：《环境科学与技术》2015年第1期1-5,共5页Environmental Science & Technology

基　　金：国家"863"计划项目(2013AA06A210);国家水污染治理重大专项项目资助(2012ZX07301);清华大学自主科研计划资助(20121087922)

摘　　要：该实验模拟污泥水解酸化出水有机酸,通过饱饥驯化法,苏丹黑和尼罗蓝染色初筛,傅立叶变换红外光谱法(FT-IR)复筛,得到一株能以城市污泥水解酸化出水混合有机酸为唯一碳源生长并积累中长链聚羟基脂肪酸酯(polyhydroxyalkanoates,PHAs)的菌株X-3。经分子生物学鉴定该菌为芽孢杆菌(Bacillus sp.)。培养实验表明碳氮比对菌株PHAs积累率有较大影响,碳氮比增高有利于菌株积累PHAs。该菌以实际污泥水解酸化液为培养基正常生长并积累PHAs。Bacterial strain X-3 was isolated from activated sludge by organic acid enrichment and carbon poor-rich domestication,Su Dan Black and Nile Blue A staining and Fourier transform infrared（FT-IR）detection. The strain can grow and accumulate PHAs utilizing mixed organic acids simulated out-of water from hydrolysis-acidification of sludge. This strain was identified as Bacillus sp by 16 S r DNA sequences analysis. C/N affected the accumulation of PHAs greatly and high C/N was favorable. Strain of Bacillus sp.X-3 can grow normally in solution from hydrolysis-acidification of sludge.

关 键 词：污泥 水解酸化 聚羟基脂肪酸酯 

分 类 号：X172[环境科学与工程—环境科学]