厌氧下Shewanella marisflavi EP1还原转化二甲戊乐灵  

Reductive Biotransformation of Pendimethalin by Shewanella Marisflavi EP1 under Anaerobic Conditions

作　　者：宁国静 李非里[1] 黄杰勋[1] 

机构地区：[1]浙江工业大学生物与环境工程学院,浙江杭州310032

出　　处：《环境科学与技术》2015年第2期19-24,共6页Environmental Science & Technology

基　　金：国家自然科学基金项目资助(21207112)

摘　　要：二甲戊乐灵是农业生产中使用量较大的一种芽前二硝基苯胺除草剂,其在环境中的持久性已经引起广泛关注。为更好地理解其在厌氧条件下的环境归趋,文章研究了Shewanella marisflavi EP1厌氧生物转化二甲戊乐灵的能力以及不同环境因子对该转化的影响。试验以乳酸钠作为电子供体,二甲戊乐灵为唯一的电子受体,EP1为还原菌,分别考察不同的反应体系和条件下EP1的生长及其对二甲戊乐灵还原的情况。结果表明,S.marisflavi EP1在厌氧条件下能有效还原转化二甲戊乐灵,核黄素的存在加快了还原反应速率,而呼吸抑制剂能够延迟甚至终止还原转化反应,表明二甲戊乐灵的还原转化是与电子传递链相关联的微生物呼吸过程。此外,EP1还原二甲戊乐灵的最优条件分别为p H 8,温度35℃,盐度2%。该研究不仅扩展了希瓦氏菌利用电子受体的潜力,也为处理硝基苯胺类农药的污染修复提供依据。Pendimethalin（PDM） is a dinitroaniline pre-emergence herbicide extensively used in agriculture, its environmental persistence has aroused wide concern. To better understand the environmental fate of PDM under anaerobic conditions, the reductive transformation of PDM by Shewanella marisflavi EP1 and the effects of various environmental factors on this transformation were evaluated. Using PDM as the sole electron acceptor, lactate as the electron donor and EP1 as degrader, the cell growth and the reduction of PDM were measured in the different systems.The results showed that the degradation of PDM by EP1 was effective under anaerobic conditions. The transformation rate increases in the presence of riboflavin, while the reduction is delayed or even completely inhibited by respiratory inhibitors, suggesting that transformation of PDM is linked to the electron transport chain of the microbial anaerobic respiration. The optimal conditions for PDM reduction by EP1 were at pH 8, 35 ℃ and 2% salinity, respectively. The findings not only expand the potential range of electron acceptor respirated by Shewanella but also provide experimental basis for dinitroaniline contaminant remediation.

关 键 词：SHEWANELLA marisflavi 二甲戊乐灵 生物转化 厌氧还原 

分 类 号：X172[环境科学与工程—环境科学]