mGM-CSF/βhCG融合蛋白的制备及其致敏树突状细胞疫苗抗小鼠RM-1前列腺癌的作用  

Preparation of mGM-CSF/βhCG fusion protein and the effect of its sensitized DC vaccine on RM-1 prostate tumor in mice

作　　者：曹荣月[1] 常娜[1] 李曼曼[1] 王云康 吴迪[1] 时宝莹 袁玉婷[1] 龙军[2] 

机构地区：[1]中国药科大学微基因药学实验室,南京210009 [2]南京中医药大学食品药品毒理学教研室,南京210023

出　　处：《中国药科大学学报》2015年第1期111-116,共6页Journal of China Pharmaceutical University

基　　金：国家自然科学基金资助项目(No.81172973;81373232);江苏高校优势学科建设工程资助项目;国家大学生创新创业训练计划资助项目(No.J1030830)~~

摘　　要：利用分子生物学方法设计并构建含有βh CG基因和m GM-CSF基因的表达载体p ET-28a-m GM-CSF-X10-βh CGCTP37质粒。乳糖诱导表达该融合蛋白,通过硫酸铵分级沉淀和阴离子交换柱DEAE-cellulose进行纯化,并以此融合蛋白致敏从C57BL/6J小鼠体内提取的树突状细胞(DC)从而获得DC疫苗。将DC疫苗回输至接种前列腺癌RM-1的C57BL/6J小鼠体内,分组检测。结果显示,DC组和DC与紫杉醇(Pac)联用(DP)组与Pac组相比抗肿瘤效果具有极显著性差异(P<0.01);且DP组的抗肿瘤效果优于DC组。可见构建的新型DC疫苗可抑制前列腺癌的生长,且与紫杉醇联合使用具有协同抗肿瘤作用。An expression vector p ET-28a-m GM-CSF-X10-βh CGCTP37 plasmid containing the βh CG and m GMCSF gene was designed and constructed. The fusion protein was induced by lactose and purified by ammonium sulfate precipitation and DEAE-cellulose anion exchange column. Then dendritic cells（ DC） in C57 BL /6J mice were extracted and sensitized by the fusion protein to obtain DC vaccine. The DC vaccine was inoculated to C57 BL of / 6J mice with prostate cancer RM-1. The results indicated that the anti-tumor effects of DC group and DC combined with paclitaxel（ DP） group were superior to that of paclitaxel（ Pac） group（ P〈0. 01）,and the anti-tumor effect of DP group was better than that of DC group. Thus,the constructed DC vaccine can inhibit the growth of prostate cancer,and have synergistic anti-tumor when used with paclitaxel.

关 键 词：ΒHCG MGM-CSF 抗肿瘤 前列腺癌 融合蛋白 免疫治疗 树突状细胞 

分 类 号：R737.25[医药卫生—肿瘤]