铁与钛离子注入对杉木幼苗叶绿素荧光参数及叶绿素含量的影响  

A comparative study on chlorophyll fluorescence and chlorophyll content characteristics effects of Ti^+ and Fe^+ ion implantation in Chinese fir seedling

作　　者：郝杰[1,2] 阮少宁[1] 林思祖[1] 张涛[3] 薛明瑜 丁恒先 

机构地区：[1]福建农林大学林学院杉木工程技术研究中心,福州350002 [2]曲靖市林业局,曲靖655000 [3]北京师范大学核科学与技术学院,北京100875

出　　处：《辐射研究与辐射工艺学报》2015年第1期58-64,共7页Journal of Radiation Research and Radiation Processing

基　　金：福建省自然科学基金项目(26121065)资助

摘　　要：用能量50 ke V的Fe+与Ti+,以不同注量D1(1.0×1015 cm-2)、D2(5.0×1015 cm-2)、D3(1.0×1016 cm-2)和D4(5.0×1016 cm-2)处理杉木种子,并采用丙酮浸泡提取法测定幼苗叶绿素含量,荧光测定仪对荧光参数进行测定。结果显示,Fe+注入较Ti+注入对初始荧光值(Fi)及可变荧光(Fv)的影响更为显著,导致叶片的Fi和Fv上升,而Ti+处理中Fi和Fv与对照比较变化不大。两种离子注入处理中PSII原初光能转化效率(Fv/Fmax)和PSII潜在的光化学活性(Fv/Fi)与对照组相比均没有明显差异。随注量的增大,Fe+处理下幼苗受到光抑制,荧光光化学淬灭系数qP曲线低于对照组而非光化学淬灭qN曲线均高于对照组,Ti+注入在D1、D2和D3时对幼苗光合具有促进作用,D1时最佳。Fe+注入导致叶绿素含量降低,叶绿素a/b值升高,显示出对叶绿素产生的抑制作用;相反Ti+注入对叶绿素含量具有一定的促进作用,可提高光合作用的能力。The effects of ion-implantation on the chlorophyll fluorescence parameters and chlorophyll content in young leaves of Chinese fir （Cunninghamia Lanceolata （Lamb.） Hook） were investigated in this paper by using 50 keV energy ion beam with different doses of Fe＋ and Ti＋ implanted into seeds, Chlorophyll fluorescence characteristics and Chlorophyll content of the seedlings were measured, the results showed that the fluorescence value （Fi） and Maximal fluorescence （Fv） were significantly higher in Fe＋ implantation as compared with Ti＋ implantation, while the Fi and Fv values were not altered by Ti＋ treatment compared with the control. Moreover, the values of Fv/Fmax and Fv/Fi in both ion-implantation treatments exhibited an increasing trend, the significance is not obvious, and Fv/Fmax value did not vary significantly. The photoinhibition phenomenon in seedlings was observed as the dose of Fe＋ implantation increased, and the qp value decreased, whereas the qN curves were significantly higher than control. A stimulating effect of Ti＋ implantation on plants＇ photosynthesis was found and the promotion effect reached maximum at D1. Fe＋ implantation decreased the chlorophyll content while increased the chlorophyll aYo value, and inhibited the synthesis of chlorophyll. By contrast, a stimulating effect of Ti＋ implantation on chlorophyll content was found, which enhanced plants＇ photosynthetic capacity.

关 键 词：杉木 离子注入 叶绿素荧光淬灭参数 叶绿素含量 

分 类 号：S722.3[农业科学—林木遗传育种] TL99[农业科学—林学]