杜仲叶黄酮苷元水解及富集纯化工艺研究  

Hydrolyzing Flavonoids and Enriching Flavonoid Aglycones from Leaves of Eucommia ulmoides Oliv.

作　　者：王志宏[1] 彭胜[1] 雷明盛[1,2,3] 郑阳[1] 史丽娟[1] 周云雷[1] 彭密军[1] 

机构地区：[1]吉首大学林产化工工程湖南省重点实验室杜仲湖南省工程实验室,张家界427000 [2]武汉大学中南医院,武汉430071 [3]湖南省张家界市人民医院,张家界427000

出　　处：《天然产物研究与开发》2015年第2期289-293,共5页Natural Product Research and Development

基　　金：国家留学基金;湖南省教育厅高校产业化培育项目(11CY015;12CY014);湖南省教育厅项目(13C758);吉首大学林产化工工程湖南省重点实验室开放项目(JDLC201208);吉首大学校级科研项目(Jd12040)

摘　　要：以杜仲叶提取物为原料,采用酸水解的方法制备杜仲叶黄酮苷元,通过单因素及正交试验优化杜仲叶黄酮苷元的水解条件;并研究了聚酰胺两步法富集黄酮苷元的工艺条件。结果表明:当料液比为2∶1(mg/m L)时,杜仲叶黄酮苷元水解的最佳工艺参数为:水解温度80℃,水解时间3 h,盐酸浓度6 mol/L,黄酮苷元的含量可达到2.512%。水解样品经过一次聚酰胺静态吸附,黄酮苷元的纯度可达12.87%,再经聚酰胺二次富集,杜仲黄酮苷元纯度可达63.19%。The preparation of flavonoid aglycone from the extract of Eucommia ulmoides Oliv. leaves was studied using acid hydrolysis. The effects of solid-liquid ratio, hydrolysis temperature, hydrolysis time and concentration of hydrochloric acid were investigated by single factor and orthogonaI experiments. Based on the optimized condition, the two-step enrich- ment technology of flavonold aglycones was studied. The results in,cared that the optimal conditions were temperature 80 ℃ ,hydrolysis for 3 h,6 mol/L of hydrochloric acid,when the solid-liquid ratio was 2：1 （mg/mL）. Under this condi- tion,the contents of flavonold aglycones reached to 2.512%. The purity of flavonoid aglyconcs increased to 12.87% af- ter static adsorption by polyamide resin. Finally,the purity of flavonoids glycosides can reach up to 63.19% through the two-step of enriching by polyamlde resin.

关 键 词：杜仲叶 黄酮苷元 水解 聚酰胺 富集 

分 类 号：TQ461[化学工程—制药化工] R284.2[医药卫生—中药学]