异粘蛋白MTDH通过上皮质间转化EMT促进胃癌的转移  

Metadherin promotes gastric carcinoma metastasis through induction of epithelial-mesenchymal transition

作　　者：夏林[1] 余国政[2] 曾宪涛[3] 李胜[3] 邴运韬[2] 李婷[2] 杨桂芳[4] 

机构地区：[1]黄石市中心医院普爱院区肿瘤内科,黄石435000 [2]武汉大学中南医院普外科 [3]武汉大学中南医院循证与转化医学中心 [4]武汉大学中南医院病理科

出　　处：《实用肿瘤学杂志》2015年第1期39-43,共5页Practical Oncology Journal

摘　　要：目的 研究异粘蛋白（MTDH）在胃癌的表达及在胃癌转移中的作用。方法 收集临床胃癌标本及培养胃癌细胞系,采用Western blot和Real-time PCR的方法检测组织中MTDH蛋白与mRNA表达水平及与EMT的关系。结果 胃癌组织中187例（86%）MTDH呈阳性表达,正常胃黏膜组织中29例（13%）呈阳性表达,MTDH在胃癌中的表达水平比正常组织高。MTDH蛋白表达与胃癌患者的TNM分期、血管浸润、肿瘤复发和转移有关（P＜0.05）。MTDH的表达与EMT过程的两个标记物相关（E-cadherin和N-cadherin）。结论 MTDH可以通过诱导EMT过程促进胃癌转移,可以作为临床治疗的一个靶基因。Objective To explore the relationship between gene MTDH expression and its role in promoting gastric carcinoma metastasis.Methods We collected clinical specimens and cultured gastric carcinoma cell lines.By Western blotting and Real-time PCR methods,protein and mRNA levels in tissues and MTDH relationship with EMT were detected.Results There was 86% of patients who expressed MTDH positively and 13% of normal gastric mucosa was positive expression.The Results showed that the expressive level of MTDH gene in gastric carcinoma was higher than in the normal tissues.The expression of MTDH was correlated with TNM stage、microvascular invasion、recurrence and metastasis.The expressive level of MTDH was correlated with two epithelial mesenchymal transition markers（E-cadherin and N-cadherin）.Conclusion MTDH may promote gastric carcinoma metastasis through the induction of EMT process and may be a candidate biomarker for therapeutic target.

关 键 词：异粘蛋白 上皮质间转化 胃癌 

分 类 号：R735.2[医药卫生—肿瘤]