地稔凝集素的提取及凝血活性的研究  

Study on the Extraction and Agglutination of Lectin from Melastoma dodecandrum Lour

作　　者：邓政东[1] 程爱芳[1] 李秀丽[2] 

机构地区：[1]武汉生物工程学院生命科学与技术学院,湖北武汉430415 [2]福建师范大学生命科学学院,福建福州350108

出　　处：《黑龙江农业科学》2015年第2期56-58,共3页Heilongjiang Agricultural Sciences

基　　金：武汉生物工程学院资助项目(2010J07)

摘　　要：为研究和利用地稔的促凝血活性物质,以地稔为试材,提取地稔凝集素,研究pH、温度和糖等理化因素对凝血活性的影响。结果表明:经硫酸铵沉淀及透析提取的地稔凝集素具有红细胞凝集活性;碱性条件下,抑制作用明显,而酸性条件有较强促进作用;温度对凝集素凝血活性影响很小;半乳糖有明显促进作用。In order to study and utilize the procoagulant activity substances from Melastoma dodecandrum Lour ,taking Melastoma dodecandrum Lour as material ,extraction of lectin and the effect of physical and chem‐ical agents on agglutinating activity were studied ,such as pH ,temperature and sugar .The results showed that the lectin extracted by ammonium sulfate precipitation and dialysis had agglutinating activity .Alkaline condi‐tions had significantly inhibited the agglutinating activity .While acidic conditions had a strong role in promo‐ting .The effect of temperature on agglutinating activity was little .Galactose had an obvious effect on promo‐ting .

关 键 词：地稔 凝集素 提取 凝血活性 

分 类 号：R284[医药卫生—中药学] R285[医药卫生—中医学]