载锌沸石对肉鸡生产性能、抗氧化功能及其相关基因表达的影响  

Effects of Zinc- Bearing Clinoptilolite on Growth Performance,Antioxidant Function and Genes Expression of Broiler Chickens

作　　者：陈刚耀[1] 唐志刚[1] 乔丽红[1] 李春梅[1] 周岩民[1] 

机构地区：[1]南京农业大学动物科技学院,南京210095

出　　处：《中国粮油学报》2015年第2期74-79,共6页Journal of the Chinese Cereals and Oils Association

摘　　要：研究载锌沸石(Zn CP,锌含量为15.5 mg/g)对肉鸡生产性能、肝脏锌沉积、抗氧化功能及其相关基因表达的影响,探讨Zn CP作为抗生素替代品的可行性。300只1日龄AA(Arbor Acres)肉鸡随机分成5组,每组6个重复,每个重复10只鸡,试验期为42 d。试验日粮:1)对照组,基础日粮(含Zn SO4,锌含量为60mg/kg);2)基础日粮+抗生素(40 mg/kg金霉素);3)基础日粮+0.1%Zn CP;4)基础日粮+0.2%Zn CP;5)基础日粮+0.4%Zn CP。结果表明,与对照组相比,不同剂量Zn CP显著提高肉鸡前期体增重(P<0.05),0.1%和0.2%的Zn CP显著提高21 d肝脏T-SOD和Cu Zn-SOD的活性(P<0.05),0.2%的Zn CP显著降低21 d肝脏MDA含量(P<0.05),抗生素组和Zn CP组均能显著提高21 d肝脏T-AOC(P<0.05);与抗生素组相比,0.1%和0.2%的Zn CP显著降低21 d肝脏MDA含量(P<0.05),显著提高21 d肝脏T-SOD的活性(P<0.05),0.1%的Zn CP显著提高42 d肉鸡肝脏Nrf2 mRNA的相对表达量(P<0.05)。与对照组和抗生素组相比,0.2%的Zn CP显著降低42 d肉鸡肝脏MDA含量(P<0.05)。结果提示,肉鸡日粮中添加Zn CP能提高生产性能,效果等同或优于抗生素;Zn CP可能通过提高Keap1/Nrf2/ARE/抗氧化酶信号通路关键基因Nrf2的表达量来提高肉鸡的抗氧化能力;本试验条件下,日粮中Zn CP以0.1%和0.2%的添加量较好。The paper has conducted to evaluate the effect of zinc - bearing clinoptilolite （ZnCP, containing Zn 15.5 mg/g） addition for its replacing antibiotics on growth performance, as well as the content in liver, antioxidant function and relevant genes expression of broiler chickens. 300 one - day - old Arbor Acres broiler chickens were randomly divided into 5 groups with 6 replicates of 10 chickens for a 42 - d feeding trial. The dietary treatments were as follows ： 1 ） control fed a basal diet with 60 mg/kg zinc from ZnSO4 ; 2） the basal diet plus antibiotics （ ANT, 40 mg chlortetracycline per kg of diet） ; 3） the basal diet plus 0.1% ZnCP; 4） the basal diet plus 0.2% ZnCP; 5） the basal diet plus 0.4% ZnCP. The results showed that compared to control, ZnCP treatments caused positive effects on BWG （P 〈 0.05 ） of broilers chickens within 1 ～21 d. The activities of T -SOD and Cu -Zn SOD in liver increased by 0.1% and 0.2% ZnCP respectively at 21 d （P 〈0.05）. MDA contents in liver on 21 d were reduced by 0.2% ZnCP （P 〈 0.05）. ANT and ZnCP treatments increased T- AOC in liver on 21 d （P 〈0.05）. Compared to ANT,0.1% and 0.2% ZnCP reduced MDA contents （P 〈0.05） and increased T - SOD activity in liver on 21 d （P 〈 0.05 ）. Nrf2 mRNA level in liver raised by 0.1% ZnCP on 42 d （P 〈 0.05 ）. Compared to control and ANT, 0.2% ZnCP improved MDA contents in liver on 42 d （P 〈0.05）. The results indicated that addition of 0.1% or 0.2% ZnCP could improve the growth performance of broiler chickens, and the effect was equivalent to or better than that of the antibiotics. ZnCP can improve antioxidant ability of broiler chickens by elevating the Nrf2 mRNA expression of Keapl/Nrf2/ARE/signaling pathway.

关 键 词：载锌沸石 肉鸡 生产性能 抗氧化功能 基因表达 

分 类 号：S815[农业科学—畜牧学]