高效液相色谱法测定鲜枣中糖的组成  

Determination of saccharides components in fresh jujube by HPLC

作　　者：孙涛[1] 赵子刚[1] 刘圣红[1] 古丽斯坦[1] 刘河疆[1] 王成[1] 

机构地区：[1]新疆农业科学院农业质量标准与检测技术研究所/农业部农产品质量安全风险评估实验室(乌鲁木齐),乌鲁木齐830091

出　　处：《分析仪器》2015年第1期38-41,共4页Analytical Instrumentation

摘　　要：研究了鲜枣中糖组成的高效液相色谱测定方法。样品经水超声提取,以Kromasil 100-5NH_2(150×4.6mm)液相色谱柱分离,Waters 2414示差检测器进行检测。结果表明5种糖在0.1~10mg/mL范围内线性良好(r≥0.9992)。平均加标回收率在87.4%~96.2%之间;相对标准偏差RSD(%)≤3.8%。该方法的最低检测限(%)范围为8.56×10^(-3)~1.53×10^(-2)。该方法简便、快速、灵敏,为鲜枣中糖组成分析提供了一种高效快速的测定方法。Saccharides were extracted from samples with water and an ultrasonic extraction process.Sucrose,glucose,fructose,maltose and lactose were separated on a Kromasil 100-5NH2 column（150×4.6mm）,analysed by Waters 2414 refractive index detector.The linear range was 0.1-10mg/mL,the correlation coefficients was ≥0.9992.The average recoveries ranged from 87.4% to 96.2%,and the relative standard deviation was ≤3.8%.The results indicate that the method is easier,faster and more sensitive.

关 键 词：高效液相色谱法 鲜枣 糖 

分 类 号：O657.72[理学—分析化学]