门静脉动脉化对肝硬化大鼠肝再生的影响  

Impacts of portal vein arterialization on liver regeneration in rats with liver cirrhosis

作　　者：蔡潮农[1] 苏永辉[1] 方瑞君[2] 李坚[1] 李培平[1] 关晓东[1] 张百萌[1] 杨禄坤[3] 

机构地区：[1]中山大学附属第五医院普通外科,珠海519000 [2]中山大学附属第五医院校区门诊,珠海519000 [3]中山大学附属第五医院麻醉科,珠海519000

出　　处：《中华肝脏外科手术学电子杂志》2014年第6期40-43,共4页Chinese Journal of Hepatic Surgery(Electronic Edition)

基　　金：珠海市科技局医药卫生重大项目(PB20081002)

摘　　要：目的探讨门静脉动脉化(PVA)对肝硬化大鼠肝再生的影响。方法取50只肝硬化模型大鼠,按随机数字表法随机分为PVA组(40只)和对照组(10只)。PVA组行PVA+门-腔静脉分流术,对照组未行任何处理。检测每组大鼠术后或入组后1周(T1)、2周(T2)、4周(T3)、8周(T4)4个时间点的肝脏湿重与体重比、增殖细胞核抗原(PCNA)阳性肝细胞百分率、DNA合成期(S期)肝细胞百分率。组内各时间点比较采用单因素方差分析,两两比较采用LSD-t检验,两组间比较采用t检验。结果 PVA组大鼠T1、T2、T3、T4时间点的肝脏湿重与体重比分别为(3.72±0.26)%、(3.81±0.27)%、(3.83±0.31)%、(3.78±0.31)%,对照组为(2.84±0.37)%,PVA组较对照组明显增大(t=6.11,6.64,6.49,6.17;P<0.05)。PVA组T1、T2、T3、T4时间点的PCNA阳性肝细胞百分率分别为(76±6)%、(69±8)%、(20±5)%、(15±4)%,对照组为(11±2)%,PVA组较对照组明显增大(t=34.48,22.87,5.69,2.93;P<0.05)。随着时间的延长,PVA组内各时间点的PCNA阳性肝细胞百分率逐渐下降,差异有统计学意义(F=316.20,P<0.05)。PVA组T1、T2、T3、T4时间点的S期肝细胞百分率分别为(27.0±1.2)%、(20.5±1.4)%、(16.2±1.3)%、(13.5±1.3)%,对照组为(11.6±1.9)%,PVA组较对照组明显增大(t=21.97,12.15,6.30,2.68;P<0.05)。随着时间的延长,PVA组内各时间点的S期肝细胞百分率逐渐下降,差异有统计学意义(F=208.00,P<0.05)。结论 PVA能有效促进肝硬化大鼠肝再生,该效应随着时间的延长而下降。ObjectiveTo investigate the impacts of portal vein arterializations （PVA） on the liver regeneration in rats with liver cirrhosis.MethodsFifty rats with liver cirrhosis model were randomly divided into PVA group （n=40） and control group （n=10） according to the random number table method. Rats in PVA group underwent PVA＋portocaval shunt. Rats in control group received no treatments. The ratio of hepatic wet weight to body weight, the hepatocyte percentage of positive proliferating cell nuclear antigen （PCNA） and hepatocyte percentage in DNA synthesis phase （S phase） in each group were measured at the time of 1 week （T1）, 2 weeks （T2）, 4 weeks （T3） and 8 weeks （T4） after operation or enrolling in the group.＆nbsp;Parameters of every time points inside the group were compared by one-way analysis of variance and pairwise comparison was conducted by LSD-t test. Comparison between groups was conducted byt test.Results The ratio of hepatic wet weight to body weight at T1,T2,T3,T4 in PVA group [（3.72±0.26）%, （3.81±0.27）%, （3.83±0.31）%, （3.78±0.31）%] were signiifcantly increased compared with that in control group [（2.84±0.37）%] （t=6.11,6.64,6.49,6.17;P〈0.05）. The hepatocyte percentage of positive PCNA at T1, T2, T3, T4 in PVA group [（76±6）%, （69±8）%, （20±5）%, （15±4）% ] were signiifcantly increased compared with that in control group [（11±2）%] （t=34.48, 22.87,5.69,2.93;P〈0.05）. The hepatocyte percentage of positive PCNA at different time points inside the PVA group decreased gradually as time extended, where signiifcant difference was observed （F=316.20,P〈0.05）. The hepatocyte percentage in S phase at T1, T2, T3, T4 in PVA group [（27.0±1.2）%, （20.5±1.4）%, （16.2±1.3）%, （13.5±1.3）%] were signiifcantly increased compared with that in control group [（11.6±1.9）%] （t=21.97, 12.15, 6.30, 2.68;P〈0.05）. The hepatocyte percentage in S phase at different time points inside the PVA group decreased g

关 键 词：肝硬化 实验性 门静脉动脉化 门腔分流术 外科 肝再生 大鼠 

分 类 号：R575.2[医药卫生—消化系统]