5种结核杆菌检测方法的临床应用价值  

The clinical value of 5 kinds of mycobacterium tuberculosis detection method

作　　者：王震[1] 龚玉华 钱彩娣 孙春红 王雪梅 施瑜[1] 周丽萍[3] 傅行礼[3] 邵启祥[3] 

机构地区：[1]江苏省镇江市第二人民医院检验科,212000 [2]江苏省镇江市第三人民医院检验科,212000 [3]江苏大学医学技术学院,江苏镇江212002

出　　处：《检验医学与临床》2015年第3期334-336,共3页Laboratory Medicine and Clinic

基　　金：江苏省镇江市社会发展基金资助项目(SH2010019)

摘　　要：目的比较和分析免疫磁珠捕获联合双内标聚合酶链反应-酶联免疫吸附试验定量检测结核杆菌技术(IMC-PCR-ELISA)与其他几种结核杆菌检测法在结核病诊断中的临床应用价值。方法以102例结核分枝杆菌痰培养阳性的患者作为阳性对照,以48例结核分枝杆菌培养阴性的非结核普通住院患者作为阴性对照。比较抗酸染色涂片法(AFS)、免疫磁珠-抗酸染色涂片法(IMC-AFS)、金标抗结核抗体检测法(DICA)、普通PCR及IMCPCR-ELISA的敏感性及特异性。结果 IMC-PCR-ELISA定量检测结核分枝杆菌全过程约4.5h,最低检测限为5copy/μL,本法的特异性和敏感性均为100.00%,其他4种(AFS、IMC-AFS、DICA、普通PCR)检测方法的敏感性分别为35.29%、43.14%、60.78%、93.14%,特异性分别为95.83%、95.83%、75.00%、91.67%。结论 IMC-PCRELISA与AFS、IMC-AFS、DICA及普通PCR相比,具有快速、灵敏、特异、可定量的特点,是临床快速诊断结核病的有效检测方法。Objective To analyze and compare the detection method for tuberculosis by immunomagnetic beads capture combined with PCR‐ELISA with double internal standard （IMC‐PCR‐ELISA） and other testing methods, and discuss its clinic application. Methods To tally 102 patients with culture‐positive tuberculosis from sputum were used as positive control, 48 nontuberculosis inpatients with culture‐negative tuberculosis from sputum were used as negative control. And the sensitivity and specificity of smear acid‐fast stain （AFS）, immune magnetic beads smear acid‐fast stain method （IMC‐AFS）, jinbiao anti‐tuberculosis antibody assay （DICA）, ordinary PCR and IMC‐PCR‐ELISA were compared. Results The results showed that the IMC‐PCR‐ELISA could yield quantitative results within about 4. 5 h with a detection limit at 5 copy/μL, the specificity and sensitivity of this method were all 100. 00%. The sensitivity of the AFS, IMC‐AFS, DICA and ordinary PCR were 35. 29%, 43. 14%, 60. 78% and 93. 14%, while the specificity were 95. 83%, 95. 83%, 75. 00% and 91. 67%, respectively. Conclusion Compared with AFS, IMC‐AFS, DICA and ordinary PCR, the IMC‐PCR‐ELISA is rapid, sensitive, secific and quantitative method for detecting tuber‐culosis, which can be taken as a helpful method for rapid detection of TB.

关 键 词：结核分枝杆菌 免疫磁珠 聚合酶链反应-酶联免疫吸附试验 检测方法 

分 类 号：R737.9[医药卫生—肿瘤]