两株高效耐盐菌的分离鉴定及生长特性研究  

Isolation, Identification and Characterization of Two Efficient Salt-tolerant Bacteria

作　　者：单德鑫[1] 李海统 黄河[1] 李健[1] 

机构地区：[1]东北农业大学资源与环境学院,哈尔滨150030

出　　处：《湖北农业科学》2014年第22期5395-5397,5407,共4页Hubei Agricultural Sciences

基　　金：黑龙江省教育厅面上项目(12521028)

摘　　要：从大庆不同利用类型的盐碱土地区取土样,经驯化、分离筛选出两株高效耐盐菌,分别命名为A和B。经形态特征观察、生理生化试验、扫描电镜观察和16S r DNA鉴定得知,菌种A为脱氮嗜盐芽孢杆菌（Virgibacillus halodenitrificans strain DSM 10037）,菌种B为咸鱼芽孢杆菌（Piscibacillus salipiscarius strain RBU1-1）,均为芽孢杆菌属。经过单因素试验,得出A、B的最适生长条件均为温度30℃,pH 7-9,盐浓度20%,这将为以后该地区微生物资源开发利用提供理论依据。Soil samples, collected from different types of saline-alkali soil of Daqing, China, were cultivated, isolated and screened. Two effective salt-tolerant bacteria strains named as A and B were obtained. The results of physiological and biochemical tests, microscopy scanning and 16 S r DNA identifation showed that the strain A was denitrogen and halophilic Bacillus（Virgibacillus halodenitrificans of strain DSM 10037）. Strain B was salted fish Bacillus（Piscibacillus salipiscarius strain RBU1-1）. Both of them were belonged to Bacillus spp. Results of one-factor test showed that the optimal temperature, pH,and salt concentration of the A and B were 30 ℃,7-9, and 20%, respectively. It will provide theoretical basis for developing and utilizing microbial resources in this region.

关 键 词：盐碱土 高效耐盐菌 分离 分子鉴定 生长特性 

分 类 号：X172[环境科学与工程—环境科学]