苎麻cpSSR标记筛选及在亲缘关系研究中的应用  

Screening cp SSR of Boehmeria(L.) Gaudich. and Its Application in Studying Genetic Relationship

作　　者：陈平[1] 喻春明[1] 王延周[1] 陈继康[1] 卢凌霄[1] 熊和平[1] 

机构地区：[1]中国农业科学院麻类研究所,长沙410205

出　　处：《湖北农业科学》2014年第21期5084-5086,共3页Hubei Agricultural Sciences

基　　金：中国农业科学院基本科研业务费(1610032012030)

摘　　要：利用cp SSR引物(叶绿体微卫星)分子标记对苎麻[(Boehmeria nivea(L.)Gaudich.]属植物进行亲缘关系分析。从22对cp SSR引物中,筛选出5对在苎麻中扩增良好、条带清晰、重复性好的引物,多态性比例为22.73%。5对引物在8份供试材料中共扩增出16条多态条带,平均每对引物的多态性条带为3.2条。聚类分析将这些材料分为3类,分类结果与采用叶绿体基因序列分类结果一致。The genetic characteristics of Boehmeria （L.） Gaudich.were analyzed with cpSSR （chloroplast microsatellites） markers.5 cpSSR primers with excellent amplification result,good repeatability and clear amplification were selected out of 22 pairs （polymorphism rate 22.73％）.16 polymorphic loci were detected from 8 tested varieties,with an average of 3.2 loci.The tested varieties were classified into 3 groups.The result was consistent with the result of analyzing chloroplast gene sequence.

关 键 词：苎麻[(Boehmeria nivea (L.) Gaudich.] 亲缘关系 CPSSR 

分 类 号：S563.1[农业科学—作物学]