Fabrication of Dex-Loaded Shape Memory Polymer Based Composite Nanofibers for Potential Bone Tissue Engineering  

作　　者：王先流 谢静 杨亮亮 娄向新 张彦中 

机构地区：[1]Laboratory of Biomimetic Biomaterials for Regenerative Medicine ,Department of Bioengineering,College of Chemistry,Chemical Engineering & Biotechnology,Donghua University

出　　处：《Journal of Donghua University(English Edition)》2014年第5期709-713,共5页东华大学学报（英文版）

基　　金：the Fundamental Research Funds for the Central Universities,China(No.14D110519);Pujiang Talent Program Funded by the Science and Technology Commission of Shanghai Municipality,China(No.10PJ1400200);National Natural Science Foundation of China(No.51073032)

摘　　要：Biodegradable shape memory polymers( SMPs) are a class of intelligent materials with great potential for imparting biomaterial scaffolds multifunctionality in the field of tissue engineering and regenerative medicine.In this study,the biodegradable SMP poly( D,L-lactide-co-trimethylene carbonate)( PLMC) incorporated with the dexamethasone( Dex),which was a kind of synthetic bone-formation inducing factor,was fabricated into nanofibers via electrospinning.The morphology,constituent,thermal and mechanical properties of the produced Dex / PLMC composite nanofibers were characterized by scanning electron microscopy( SEM), Fourier transform infrared spectroscopy( FTIR), differential scanning calorimetry( DSC),and tensile testing,respectively.Then,ultrasound was employed as a remote stimulus to regulate the Dex releasing behavior from the composite nanofibers.It was found that the generated Dex /PLMC composite nanofibers had a uniform and smooth morphology with a diameter of ca.564 nm.Mechanical testing results showed that incorporation of the Dex gave rise to improved mechanical performance with the tensile strength,Young's modulus and strainat-break increased by 18.2%,20.0% and 64.4%,respectively.DSC data revealed that the glass transition temperature( Tg) of the composite nanofibers, i.e., the thermal transition temperature( Ttrans) for activating shape memory effect, was 39.7 ℃.Moreover, the release kinetics of the encapsulated Dex in the nanofibers could be manipulated by varying the acoustic power and insonation duration.These results suggested that the newly developed Dex / PLMC nanofibers could be a promising drug delivery system for applications in bone tissue engineering( BTE).Biodegradable shape memory polymers( SMPs) are a class of intelligent materials with great potential for imparting biomaterial scaffolds multifunctionality in the field of tissue engineering and regenerative medicine.In this study,the biodegradable SMP poly( D,L-lactide-co-trimethylene carbonate)( PLMC) incorporated with the dexamethasone( Dex),which was a kind of synthetic bone-formation inducing factor,was fabricated into nanofibers via electrospinning.The morphology,constituent,thermal and mechanical properties of the produced Dex / PLMC composite nanofibers were characterized by scanning electron microscopy( SEM), Fourier transform infrared spectroscopy( FTIR), differential scanning calorimetry( DSC),and tensile testing,respectively.Then,ultrasound was employed as a remote stimulus to regulate the Dex releasing behavior from the composite nanofibers.It was found that the generated Dex /PLMC composite nanofibers had a uniform and smooth morphology with a diameter of ca.564 nm.Mechanical testing results showed that incorporation of the Dex gave rise to improved mechanical performance with the tensile strength,Young's modulus and strainat-break increased by 18.2%,20.0% and 64.4%,respectively.DSC data revealed that the glass transition temperature( Tg) of the composite nanofibers, i.e., the thermal transition temperature( Ttrans) for activating shape memory effect, was 39.7 ℃.Moreover, the release kinetics of the encapsulated Dex in the nanofibers could be manipulated by varying the acoustic power and insonation duration.These results suggested that the newly developed Dex / PLMC nanofibers could be a promising drug delivery system for applications in bone tissue engineering( BTE).

关 键 词：DEXAMETHASONE (Dex) shape memory polymer (SMP) ultrasound BONE TISSUE engineering( BTE) ELECTROSPINNING 

分 类 号：O623.6[理学—有机化学]