认知功能损害与ApoE基因多态性的关联性研究  

An association study on polymorphisms of Apolipoprotein E gene and mild cognitive impairment

作　　者：王志强[1] 张付全[1] 陈新宇[1] 董昆仑[1] 王栋[1] 

机构地区：[1]江苏省无锡市精神卫生中心,214000

出　　处：《精神医学杂志》2014年第6期413-416,共4页Journal of Psychiatry

基　　金：国家自然科学基金资助项目(编号:30670714);江苏省自然科学基金项目(编号:BK2006028)

摘　　要：目的探讨认知功能损害与载脂蛋白E(Apo E)基因多态性的相关性。方法采集46例正常对照(对照组)、67例轻度认知功能损害患者(MCI组)和42例AD痴呆期患者(DAT组)的外周血提取DNA,采用聚合酶链反应-限制性片段长度多态技术(PCR-RFLP)测定上述3组人群中Apo E等位基因的分布频率。结果 3种等位基因在对照组、MCI组和DAT组中的总体分布差异有统计学意义(P=0.018)。ε2和ε3等位基因频率在3组间的分布差异无统计学意义(P=0.373,P=0.302),但DAT组ε4等位基因的频率显著高于对照组(P=0.011)和MCI组(P=0.019),ε4等位基因的频率在对照组和MCI组之间的差异无统计学意义(P=0.742)。结论 Apo Eε4等位基因可能在DAT发病中发挥一定作用,但其与MCI的发生可能不具有关联性。Objective To explore the association between the polymorphisms of Apolipoprotein E （ApoE） gene and cognitive impairment.Methods DNA was isolated from peripheral blood of 46 healthy controls （control group）,67 subjects with mild cognitive impairment （MCI group） and 42 subjects with dementia with alzheimer type （DAT group）.Polymorphisms of ApoE were detected by using polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP）.Results There was significant difference in the overall distribution of ApoE alleles among the three groups （P =0.018）.There were no significant differences in the distribution of ε2 and ε3 alleles among the three groups （P =0.373,0.302 respectively）.However,the frequency of ApoE ε4 was significantly higher in DAT group than that in control group （P =0.011） and MCI group （P =0.019）.There was no significant difference in the frequency of ApoE ε4 alleles between control group and MCI group （P =0.742）.Conclusion ApoE ε4 allele may play a role in the development of DAT,but it is unlikely to be associated with MCI.

关 键 词：Alzheimer’s病 轻度认知功能损害 载脂蛋白E 关联 

分 类 号：R749.1[医药卫生—神经病学与精神病学]