原花青素对肢体缺血再灌注后大鼠肠黏膜屏障功能的保护作用  

Effects of proanthocyanidin on intestinal mucosal barrier after limb ischemia reperfusion injury in rats

作　　者：陈建敏[1] 宋强[1] 杨拯[1] 罗兰[1] 李禹呈[1] 卓睿[1] 

机构地区：[1]成都医学院基础医学院基础医学实验教学中心,成都610500

出　　处：《山西医科大学学报》2015年第2期132-135,共4页Journal of Shanxi Medical University

基　　金：成都医学院自然科学基金项目(CYZ12-012)

摘　　要：目的探讨原花青素对大鼠肢体缺血再灌注(limb ischemia reperfusion,LIR)后肠黏膜屏障功能的保护作用。方法健康成年SD大鼠21只,随机分为三组:假手术组(n=7),肢体缺血再灌注组(n=7)和原花青素组(n=7);原花青素组给予原花青素[100 mg/(kg·d)]预处理7 d,假手术组和肢体缺血再灌注组给予等量色拉油,预处理7 d。肢体缺血再灌注组和原花青素组采用止血带结扎大鼠左后肢3 h后再灌注18 h的方法建立肢体缺血再灌注动物模型。假手术组行止血带结扎操作后立即剪断止血带。测定各组动物的血清二胺氧化酶(DAO)活性和前列环素(PGI2)含量,以及肠组织的分泌型免疫球蛋白A(sIgA)含量。结果与假手术组比较,肢体缺血再灌注组大鼠血清DAO活性显著升高(P<0.05),血清PGI2含量显著增多(P<0.01),肠组织中sIgA含量显著减少(P<0.05);与肢体缺血再灌注组比较,原花青素组大鼠血清DAO活性显著降低(P<0.05),血清PGI2含量显著减少(P<0.01),肠组织中sIgA含量显著增加(P<0.05)。结论原花青素对LIR所致的肠黏膜屏障功能损伤具有保护作用。Objective To investigate the protective effects of proanthocyanidin on the intestinal mucosal barrier after limb ischemia repeffusion（LIR） in rats. Methods Twenty-one SD rats were randomly divided into sham group （n = 7 ）, LIR group （n = 7 ）, and proanthocyanidin group（n = 7 ）. The rats in proanthocyanidin group were pretreated with proanthocyanidin at a dosage of 100 mg/ （kg · d） for 7 d before surgery, while in the other two groups the rats were pretreated with equivoluminal salad oil for 7 d. LIR model was made by ligation with tourniquet for 3 h followed by reperfusion for 18 h on the left hind limbs of the rats. The rats in sham group were treated by ligation with the tourniquet, and the tournique was cut off as soon as the ligation operation was finished. The activity of diamine oxidase（DAO） and the content of prostacyclin（ PGI2 ） in serum were measured. The content of intestinal secretory immunoglobu- lin（sIgA） was determined. Results Compared with sham group, the DAO activity and PGI2 content were increased significantly（P 〈 0.05 or 0.01 ）, while the content of sIgA decreased significantly in LIR group（P 〈0.05）. Compared with LIR group, the DAO activity and PGI2 content were decreased significantly（P 〈0.05 or 0.01 ）, while the content of slgA increased significantly in pmanthocyanidin group（P 〈0.05）. Conclusion Proanthocyanidin may play a protective role in the injury of intestinal mueosal barrier caused by LIR.

关 键 词：原花青素 肢体缺血再灌注 二胺氧化酶 前列环素 分泌型免疫球蛋白A 

分 类 号：R363[医药卫生—病理学]