水稻稻瘟病广谱抗病新等位基因pi21t的鉴定及其抗性应用  

Identification and Application of One New Rice Blast Broad-spectrum Resistance Allele pi21t

作　　者：朱金燕[1] 王军[1] 范方军[1] 李文奇[1] 王芳权[1] 仲维功[1] 杨杰[1] 

机构地区：[1]江苏省农业科学院粮食作物研究所,国家水稻改良中心南京分中心,江苏南京210014

出　　处：《华北农学报》2014年第6期11-15,共5页Acta Agriculturae Boreali-Sinica

基　　金：江苏省自然科学基金项目(BK20130725);江苏省科技支撑计划项目(BE2012309);国家重点基础研究发展计划项目(2013CBA01405);优质高产多抗广适性水稻新品种选育项目(CX(12)1003-2)

摘　　要：为不断发掘和鉴定新的抗性基因,从而克隆和利用广谱持久抗性基因,选育水稻广谱抗稻瘟病新品种,解决稻瘟病危害。根据已克隆的水稻稻瘟病广谱抗病隐性等位基因pi21的序列设计分子标记Pi21-1,对育种上高频率使用的广亲和品种02428进行PCR扩增,测序比较分析发现了新的等位基因类型,同时对广亲和品种02428进行田间稻瘟病接种鉴定,苗期鉴定结果为免疫,从而鉴定了一个广谱抗稻瘟病的新等位基因pi21t,为水稻广谱抗稻瘟病育种提供了新的有利资源。通过分子标记辅助选择,能够快速明确目标品种中所携带目标基因的等位基因型,从而加快水稻广谱抗稻瘟病育种的进程,提高抗病效率。In order to discover,identify and apply the new broad-spectrum resistant genes,this research had identified one new rice blast broad-spectrum resistant allele. In this study,according to the sequence of rice blast broad-spectrum resistance recessive allele pi21,molecular marker Pi21-1 was designed and amplified using wide compatibility rice variety 02428. Sequencing analysis showed new mutation type,and rice blast vaccination identification resulted in immunity. Thus,a new rice blast broad-spectrum resistance allele pi21 t had been identified. These results provided new resistant resources for rice blast broad-spectrum resistant breeding. Meanwhile,genotype of target variety could be determined rapidly using newly designed marker. All these accelerate the process of rice blast broad-spectrum resistant breeding.

关 键 词：水稻 稻瘟病 广谱抗病 等位基因型 

分 类 号：Q78[生物学—分子生物学] S435.11[农业科学—农业昆虫与害虫防治]