基因本体论在福氏志贺菌转录组研究中的应用  

Application of Gene Ontology Annotations in Shigella flexneri Transcriptome Study

作　　者：朱阵 刘翠翠[1] 王婧[1] 张继瑜[1] 魏小娟[1] 周绪正[1] 李冰[1] 郭肖[1] 

机构地区：[1]中国农业科学院兰州畜牧与兽药研究所,农业部兽用药物创制重点实验室,兰州730050

出　　处：《西北农业学报》2014年第12期17-24,共8页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家自然科学基金(31272603;31101836)

摘　　要：利用RNA-seq技术进行环丙沙星敏感和耐药的福氏志贺菌2457T株的序列测定与组装,对其碱基序列借助基因本体(Gene Ontology,GO)在生物进程、细胞组分、分子功能三方面进行注释和生物学分析。研究经过耐药诱导的转录组差异表达情况,并探讨差异表达基因在耐药性产生过程中的作用及相互关系。结果表明,通过耐药诱导有3 641个基因差异表达,其中961个GO条目注释到39个功能类别。同时发现硫酸盐、硝酸盐氧化还原相关酶系,电子/H+传导基因,膜转运蛋白,ABC外排泵家族4类耐药相关的功能基因组分。The sequence and assemble the transcriptome of ciprofloxacin-sensitive and-resistant Shigella flexneri 2457 Tstrain was conducted by RNA-seq and Gene Ontology was used to annotate and analyze the base sequence in biological processes,cellular components,molecular functions.After the study of drug-induced transcriptome expression differences,and the role of genes differentially expressed in the process of drug resistance and their relationship was explored.Results showed that there were 3 641 genes differentially expressed,of which 961 GO annotation entries to 39 functional categories.At the same time,drug-related functional gene components were found,including sulfates and nitrates redox-related enzymes,electronic/ H^＋spread genes,membrane transport proteins and ABC efflux pump family.

关 键 词：RNA-SEQ 福氏志贺菌 转录组 基因本论 

分 类 号：Q78[生物学—分子生物学]