PRDX1通过上皮间质转化促进结肠癌SW480细胞侵袭转移  

PRDX1 participates in invasion and metastasis of colon cancer through epithelial-mesenchymal transition

作　　者：冯继红[1] 冯冬冬 傅仲学[1] 

机构地区：[1]重庆医科大学附属第一医院胃肠外科,重庆400016 [2]安徽省六安市寿县中医院心内科,安徽六安232200

出　　处：《遵义医学院学报》2015年第1期67-73,共7页Journal of Zunyi Medical University

基　　金：国家自然科学基金面上项目(NO:81172295)

摘　　要：目的探讨过氧化还原酶1(Peroxiredoxin 1,PRDX1)在结肠癌上皮间质转化(epithelialmesenchymal transition,EMT)发生中的作用及其参与结肠癌侵袭转移的可能机制。方法通过慢病毒转染使结肠癌SW480细胞PRDX1过表达并通过Western blot检测验证;采用Transwell方法和细胞划痕实验检测细胞侵袭及转移能力的变化;免疫细胞荧光检测结肠癌SW480细胞EMT相关蛋白Twist1、E-cadherin及Vimentin的表达;Western blot方法检测EMT转录因子Twist1、EMT相关蛋白E-cadherin、N-cadherin及Vimentin及转移相关蛋白MMP-9的表达。结果转染PRDX1组SW480细胞中PRDX1呈过表达;Transwell法和细胞划痕实验显示过表达PRDX1组细胞的侵袭及转移能力较对照组明显增强;免疫细胞荧光显示转染PRDX1组细胞其上皮细胞标记E-cadherin明显下调,间质标记Vimentin及EMT转录因子Twist1表达明显上调;Western blot结果表明过表达PRDX1组E-cadherin较对照组显著降低(P<0.01),而Twist1、MMP-9、N-cadherin及Vimentin较对照组显著升高(P<0.05)。结论 PRDX1可促进人结肠癌SW480细胞EMT的发生,从而增强结肠癌SW480细胞的侵袭、转移能力。Objective To study the role of Peroxiredoxin l （PRDX1） in epithelial -mesenchymal transition in colon cancer and the mechanism of PRDX1 participating in the invasion and metastasis of colon cancer. Methods SW480 cells were transfected with lentivirus vectors resulting in overexpression of PRDX1 protein, and the ex- pression of PRDX1 were validated by western blot. Transwell migration assays and wound healing assays were performed to identify the differences and changes of invasive and metastatic ability in SW480 cell lines. The ex- pression of Twist1, E - cadherin and Vimentin were analyzed by immunocytochemistry. The expression of Twistl （EMT related transcription factor）, E -cadherin,N -cadherin and Vimentin （EMT biomarker proteins）, and MMP9 （metastasis associated protein） were detected by western blot. Results PRDX1 are over -expressed in the transfection groups, as compared to that in control groups （ P 〈 0.01 ）. The transfected cells significantly have stronger capability for invasion and metastasis as compared to those in control groups. The immunofluorescence staining revealed that the expression of E - cadherin was decreased while the expression of Vimentin and Twistl was increased in the transfected cells. Western blot showed that the expression of E - cadherin was decreased significantly in the transfection groups in compared with control group （ P 〈 0.01 ）, while the expression of Twistl, MMP - 9, N - cadherin and Vimentin was increased significantly （ P 〈 0.05 ）. Conclusion PRDX1 can induce EMT process of colon carcinoma line SW480 cells, and promote its ability of invasion and metastasis.

关 键 词：过氧化还原酶1 结肠癌 侵袭 转移 上皮间质转化 

分 类 号：R735.3[医药卫生—肿瘤]