三氧化矿物凝聚体对乳、恒牙牙髓细胞增殖和分化影响的比较  

Effects of mineral trioxide aggregate on the proliferation and differentiation of human pulp cells from primary and permanent teeth

作　　者：王敏永[1] 刘鹤[1] 李盛林 秦满[1] 

机构地区：[1]北京大学口腔医学院·口腔医院儿童口腔科 [2]口腔颌面外科研究室,北京100081

出　　处：《华西口腔医学杂志》2015年第1期75-79,共5页West China Journal of Stomatology

摘　　要：目的对比三氧化矿物凝聚体(MTA)和氢氧化钙对人乳、恒牙牙髓细胞增殖和分化的影响。方法采用甲基噻唑基四唑(MTT)法检测牙髓细胞生长增殖变化;实时荧光定量聚合酶链反应(PCR)检测碱性磷酸酶(ALP)、牙本质涎磷蛋白(DSPP)、骨保护因子(OPG)、核因子κB受体活化因子配体(RANKL)基因的表达。结果氢氧化钙组乳、恒牙牙髓细胞增殖均显著低于对照组(P<0.01),MTA组乳、恒牙牙髓细胞增殖均高于对照组(P<0.01)。实时荧光定量PCR检测结果显示,乳牙氢氧化钙组ALP、DSPP和OPG的表达显著低于对照组(P<0.01),MTA组上述因子的表达显著高于对照组(P<0.01);氢氧化钙组RANKL的表达显著高于对照组(P<0.01),MTA组RANKL的表达与对照组差异无统计学意义(P>0.05)。恒牙牙髓细胞氢氧化钙组ALP和DSPP的表达与对照组相比无显著差异(P>0.05),MTA组ALP和DSPP的表达显著增加(P<0.01);氢氧化钙组和MTA组OPG、RANKL的表达与对照组无显著差异(P>0.05)。结论 MTA比氢氧化钙更适合做乳牙和恒牙的盖髓剂,其优势在乳牙可能更为明显。Objective This study aims to investigate the effects of mineral trioxide aggregate （MTA） and calcium hydroxide on proliferation and differentiation of human pulp cells from primary and permanent teeth. Methods Cell proliferation was detected by methyl thiazolyl tetrazolium （MTT） assay. The mRNA expression levels of dentinogenesis-related factors, including alkaline phosphatase （ALP） and dentin sialophosphoprotein （DSPP）, and odontoclastogenesis-related factors, such as osteo- protegerin （OPG） and receptor activator of NF-κB ligand （RANKL）, were determined by real time polymerase chain reac- tion （PCR）. Results Primary and permanent pulp cells treated with calcium hydroxide exhibited significantly lower proli- feration rates than the control cells （P〈0.01）. By contrast, the MTA-treated group showed significantly higher proliferation rates than the control group （P〈0.01）. Real time PCR results showed that calcium hydroxide-treated primary pulp cells exhi- bited significantly decreased ALP, DSPP, and OPG expression compared with the control group （P〈0.01）. Conversely, the MTA-treated group displayed significantly increased ALP, DSPP, and OPG expression （P〈0.01）. Calcium hydroxide-treated primary pulp cells also exhibited significantly upregulated RANKL expression （P〈0.01）; by contrast, MTA-treated cells did not show any change in RANKL expression （P〉0.05）. Likewise, MTA-treated permanent pulp cells showed significantly upregulated ALP and DSPP expression （P〈0.01）. However, the calcium hydroxide-treated group remained almost the same as the control group （P〉0.05）. Neither MTA nor calcium hydroxide affected OPG and RANKL expression in per- manent pulp cells （P〉0.05）. Conclusion MTA is more suitable as a pulp-capping agent, particularly in primary teeth, than calcium hydroxide.

关 键 词：三氧化矿物凝聚体 氢氧化钙 碱性磷酸酶 牙本质涎磷蛋白 骨保护因子 核因子κB受体活化 因子配体 牙髓细胞 

分 类 号：R783.1[医药卫生—口腔医学]