股骨头坏死(ONFH)患者滑膜组织来源的类成纤维细胞对人Burkitt淋巴瘤(BL) Raji细胞增殖的影响  

The effect of fibroblast-like cells derived from the synovial membrane of the patients with osteonecrosis of femeral head(ONFH) on the proliferation of Raji cells from human Burkitt lymphoma(BL)

作　　者：王翠[1] 何崇儒[2] 吴礼杨[3] 钟扬[1] 孙庆文[1] 

机构地区：[1]复旦大学生命科学学院遗传学研究所,上海200433 [2]第二军医大学附属长海医院骨科,上海200433 [3]汕头大学医学院附属粤北人民医院骨科,韶关512026

出　　处：《复旦学报（医学版）》2015年第1期77-83,共7页Fudan University Journal of Medical Sciences

摘　　要：目的研究股骨头坏死(osteonecrosis of the femeral head,ONFH)患者滑膜组织来源的类成纤维细胞对人Burkitt淋巴瘤(Burkitt lymphoma,BL)Raji细胞株增殖的影响。方法首先分离和培养来自ONFH患者滑膜组织的原代类成纤维细胞,随后将其与Raji细胞共培养;用细胞计数试剂盒(CCK-8)检测Raji细胞的增殖情况;用实时定量PCR(quantitative real-time PCR,qRT-PCR)检测Raji细胞中P53、P21及CD9基因的表达水平。结果 ONFH滑膜来源的细胞为贴壁的类成纤维细胞。共培养组中的Raji细胞增殖速率显著高于单独培养组(只培养Raji细胞)。相对于单独培养组,共培养24h后,P53、P21及CD9的表达量分别为0.82(P<0.05)、1.75(P<0.05)及1.99(P<0.05),48h后,则分别为0.72(P<0.05)、1.03及0.56(P<0.05)。结论在共培养条件下,ONFH患者滑膜组织来源的类成纤维细胞可能通过调控Raji细胞中P53、P21及CD9的表达来促进Raji细胞的增殖。Objective To investigate the effect of fibroblast-like cells membrane of the patients with osteoneerosis of the femeral head （ONFH） from human Burkitt lymphoma （BL）. Methods Primary fibroblast-lik membrane of （）NFH patients were isolated and cultured,and then a co-cul Raji cells. The proliferation of Raji cells was determined by CCK-8 P53,P21 and CD9 mRNA in Raji cells were determined by quantitati which derived from the synovial on the proliferation of Raji cells e cells derived from the synovial ture system was established with assay, and the expressions of ve real-time PCR （qRT-PCR）.Results The cells derived from the synovial membrane of ONFH were adherent fibroblast-like cells. The proliferation rate of Raji cells in co-culture group was significantly higher than that in individual group （cultured Raji ceils alone）. Compared with the individual group, the relative expression amount of P53, P21 ,and CD9 mRNA after co-cultured for 24 hours in co-culture group was 0.82 （P〈0. 05）,1.75 （P〈0. 05） ,and 1.99 （P〈0. 05） ,respectively; and was 0. 72 （P〈0. 05）, 1.03 ,and 0. 56 （P〈0. 05）,respectively after co-cultured for 48 hours. Conclusions Under co-culture condition, the fibroblast-like cells from synovial membrane of ONFH patients could significantly promote the proliferation of Raji cells by regulating the expression of P53,P21 ,and CD9 mRNA.

关 键 词：股骨头坏死(ONFH) Burkitt淋巴瘤(BL) 共培养 RAJI细胞 

分 类 号：R73-35[医药卫生—肿瘤]