黄芪多糖对慢性心衰大鼠心肌AMPK活性和FFA代谢的影响  

Astragalus polysaccharides improve chronic heart failure by promoting myocardial FFA metabolism via AMPK pathway

作　　者：宋杰[1] 胡阳黔[1] 刘坚[2] 李静[2] 

机构地区：[1]湖北医药学院附属东风医院,湖北十堰442000 [2]湖北医药学院,湖北十堰442000

出　　处：《中国病理生理杂志》2015年第1期28-32,共5页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30370673);湖北省教育厅科学研究计划资助项目(No.B2013120;B2014062);十堰市科学技术研究与开发项目(No.ZD2012037)

摘　　要：目的:探讨黄芪多糖(APS)对慢性心力衰竭(CHF)大鼠心肌腺苷酸活化蛋白激酶(AMPK)活性和游离脂肪酸(FFA)代谢的影响。方法:32只雄性SD大鼠随机分为4组:正常对照组、假手术组、模型组和APS组,每组8只。采用大鼠左侧冠状动脉结扎术建立心肌梗死后心衰模型。造模成功后,APS组大鼠给予APS(3 g·kg-1·d-1)连续灌胃6周。采用心脏超声检测左心室舒张期内径(LVD)、左心室收缩期内径(LVS)、左心室射血分数(LVEF)和短轴缩短率(FS);HE染色观察心肌病理形态学改变;乙酰辅酶A合成酶-乙酰辅酶A氧化酶法(ACS-ACOD)检测血清及心肌FFA浓度;Western blotting法测大鼠心肌总AMPK、磷酸化AMPK(p-AMPK)、脂肪酸转位酶(FAT/CD36)和肉毒碱软脂酰转移酶-1(CPT-1)的蛋白表达情况。结果:假手术组与对照组比较各项指标无显著差异。模型组较对照组LVEF和FS显著降低(P<0.05)而LVD和LVS显著增加(P<0.05)。APS组LVEF和FS较模型组明显改善(P<0.05)并且LVD和LVS较模型组明显减小(P<0.05)。HE染色显示模型组较对照组心肌坏死灶增加,残余心肌细胞减少;而APS组较模型组心肌坏死灶减少,残余心肌细胞增多。模型组血清及心肌FFA浓度较对照组明显增加(P<0.05);APS组血清及心肌FFA浓度较模型组明显减少(P<0.05)。模型组p-AMPK、CPT-1和细胞膜FAT/CD36表达较对照组显著减少(P<0.05);而与模型组相比APS组p-AMPK、CPT-1和细胞膜FAT/CD36表达明显增加(P<0.05)。结论:APS可能通过激活慢性心衰大鼠AMPK相关通路促进心肌摄取利用FFA,从而改善慢性心衰。AIM：To investigate the effect of Astragalus polysaccharides （ APS） on chronic heart failure and its mechanism.METHODS：Male SD rats （n=32） were randomly divided into control group , sham group, model group and APS group （8 rats in each group）.The left coronary artery ligation in the rats was conducted to establish myocardial infarc -tion heart failure model.After modeling, the rats in APS group were given APS （3 g＆#183; kg-1＆#183; d-1） by intragastric adminis-tration for 6 weeks.Left ventricular diastolic diameter （LVD）, left ventricular systolic diameter （LVS）, left ventricular ejection fraction （ LVEF） and fractional shortening （ FS） were detected by echocardiography .HE staining was used to ob-serve the pathological changes .The concentrations of free fatty acid （ FFA） in the serum and myocardium were observed by the method of acetyl coenzyme A synthetase and acetyl coenzyme A oxidase （ ACS-ACOD ） .The protein levels of total AMP-activated protein kinase （AMPK）, phosphorylated AMP-activated protein kinase （p-AMPK）, fatty acid translocase （FAT/CD36） and carnitine palmitoyltransferase I （CPT-1） were measured by Western blotting.RESULTS： No signifi-cant difference in each index between sham group and control group was observed .Compared with control group , LVEF and FS in model group was significantly decreased , while LVD and LVS was significantly increased （ P〈0.05 ） .The LVEF and FS in APS group were significantly improved compared with model group （ P〈0.05 ） , and there was no significant difference between APS group and control group .LVD and LVS in APS group were obviously improved compared with mo-del group （P〈0.05）, and the difference was significant compared with control group （P〈0.05）.Compared with control group, focal myocardial necrosis increased , and residual myocardial cells reduced in model group , while those was much better in APS group as compared with model group （P〈0.05）.The FFA concentrations in th

关 键 词：黄芪多糖 游离脂肪酸 腺苷酸活化蛋白激酶 慢性心力衰竭 

分 类 号：R363[医药卫生—病理学]