DLL4及HIF-1α在乳癌组织表达及意义  

EXPRESSION OF DLL4 AND HIF-1α IN BREAST CANCER AND ITS CLINICAL SIGNIFICANCE

作　　者：郎志强[1] 吴艳秋[1] 郝俊梅[1] 封小丽[1] 潘旭波[1] 

机构地区：[1]青岛大学医学院附属烟台毓璜顶医院病理科,山东烟台264000

出　　处：《齐鲁医学杂志》2015年第1期4-6,共3页Medical Journal of Qilu

基　　金：烟台市科技发展计划资助项目(2008142-15)

摘　　要：目的 探讨Delta样配体4（DLL4）和低氧诱导因子1α（HIF-1α）在乳癌组织的表达及其临床意义。方法 采用免疫组化方法,检测122例乳房浸润性导管癌（其中Ⅰ级33例,Ⅱ级40例,Ⅲ级49例）组织中DLL4和HIF-1α的表达,分析两者表达与乳癌临床病理参数的相关性。结果 DLL4和HIF-1α在乳房浸润性导管癌低级别组（Ⅰ级、Ⅱ级）和高级别组（Ⅲ级）中的表达阳性率分别为63.0%、91.8%和56.2%、89.8%,差异有统计学意义（χ^2=12.852、15.695,P〈0.05）,但HIF-1α在Ⅰ级和Ⅱ级间的表达差异无统计学意义（P〉0.05）。DLL4在乳癌组织中的表达与组织学分级、肿瘤大小、临床分期、淋巴结转移有关（χ^2=4.802-12.852,P〈0.05）,与病人年龄、雌激素受体（ER）、孕激素受体（PR）、HER2表达无相关性（P〉0.05）。HIF-1α在乳癌组织中的表达与组织学分级和淋巴结转移相关（χ2=8.961、15.695,P〈0.05）,与病人年龄、肿瘤大小、ER、PR、HER2表达无关（P〉0.05）。乳癌DLL4阳性表达病人HIF-1α表达阳性率明显高于DLL4阴性表达病人,两者表达水平呈正相关（r=0.270,P〈0.01）。结论DLL4和HIF-1α可能共同调控乳癌肿瘤血管生成并影响乳癌的发展及预后,可作为预测乳癌预后的重要参考指标。Objective To explore the expression of Delta-like Ligand 4 （DLL4） and hypoxia-inducible factor 1α （HIF-1α） in breast cancer and its clinical significance. Methods Immunohistochemical staining was used to detect the expression of DLL4 and HIF let in 122 cases of infiltrating duct cancer of the breast-grade T 33 cases, grade Ⅱ ,40, and grade Ⅲ ,49. The correlation between the two expressions with clinieopathologie parameters was analyzed. Results The positive rate of DLL4 and HIF-1α in the low grade （grade Ⅰ and Ⅱ ） cancer group was 63.0% and 91.8%, respectively, and that in the high-grade cancer group was 56.2% and 89.8%, the differences being statistically significant （x^2 = 12.852,15.695 ;P〈0.05）. While the difference of HIF-1α expression between the grade Ⅰ and grade Ⅱ group was not significant （P〉0.05）. The expression of DLL4 in the breast cancer was associated with histological grade, tumor size, clinical stage and lymph node metastasis （x^2 =4.802-12.852 ,P〈0.05）, and unrelated with patient age, ER,PR and HER2 expression （P〉0.05）. The expression of HIF-la in patients with positive DLL4 was significantly higher than in those with negative DLL4 （x^2 =8.961,15.695 ;P〈0.05）, the two expressions were positively correlated （r=0.270, P〈0.01）. Conclusion DLL4 and HIF-1α are likely to co-regulate angiogenesis of the breast cancer and affect the development and prognosis of this malignancy, which can be used as an important reference mark for predicting its prognosis.

关 键 词：乳房肿瘤 Delta样配体4 缺氧诱导因子1 免疫组织化学 

分 类 号：R737.9[医药卫生—肿瘤]