抗肝硬化不同中药组方大鼠含药血清对肝硬化HSC-T6细胞相关基因表达的影响  

Effects of drug-containing serum of different anti-hepatic-cirrhosis prescription on related gene expression of HSC-T6cells

作　　者：崔爱玲[1] 孙晓慧[1] 刘中景[1] 于朝霞[1] 

机构地区：[1]青岛市传染病医院中西医结合科,山东青岛266033

出　　处：《中国中西医结合消化杂志》2015年第1期10-13,共4页Chinese Journal of Integrated Traditional and Western Medicine on Digestion

基　　金：青岛市中医药科研计划重点资助(No:2009-ZYZ002)

摘　　要：[目的]观察抗肝硬化不同中药组方大鼠含药血清对肝硬化HSC-T6细胞相关基因表达的影响及其效果差异。[方法]将30只SD大鼠随机分为正常对照组、秋水仙碱对照组、抗肝硬化不同中药组(1号方组、2号方组、3号方组)共5组,每组6只,HSC-T6细胞复苏后常规培养,采用半定量反转录聚合酶链反应(RT-PCR)和ELISA法观察药物对肝硬化HSC-T6细胞Ⅰ型胶原(Co1Ⅰ)、Ⅲ型胶原(Co1Ⅲ)和前凋亡蛋白(Bax)的mRNA表达的影响。[结果]与正常对照组比较,抗肝硬化不同中药1号方和2号方对Co1Ⅰ、Co1Ⅲ、Bax mRNA的表达差异均有统计学意义(P<0.05,P<0.01),而3号方除对Bax mRNA的表达与正常对照组比较差异有统计学意义(P<0.05),而其对Co1Ⅰ、Co1ⅢmRNA的表达与正常对照组比较,差异无统计学意义。[结论]抗肝硬化不同中药组方大鼠含药血清对肝硬化HSC-T6细胞相关基因表达均有不同程度的抑制作用。[Objective]To investigate influences and effects of drug-containing serum of different anti-he- patic-cirrhosis prescription on related gene expression of HSC-T6 cells. [Methods]Thirty SD rats were ran- domly divided into five groups：control group,colchicine group and three different prescription trial groups （Group 1,Group 2 and Group 3）. After the resuscitation and culture of HSC-T6 cells,the influences of dif- ferent drugs on mRNA expression of Collagen Type I,Collagen Type m and apoptosis protein Bax in HSC- T6 cells were detected by semi-quantitative reverse-transcriptase polymerase chain reaction（RT-PCR）and ELISA analysis. [Results]As compared with the control group,mRNA expression of Collagen Type I,Col- lagen Type []I and apoptosis protein Bax showed significant differences in Group 1 and Group 2（P〈0. 05; P〈0.01）. As compared with the normal control,Group 3 had significant effect on mRNA expression of ap- optosis protein Bax with no significant effect on mRNA expression of Collagen Type I （P〈0.05）. [Con- clusion]Drug-containing serum of different anti-hepatic-cirrhosis prescription had varying inhibitory effects on related gene expression of HSC-T6 cells.

关 键 词：抗肝硬化不同中药 大鼠含药血清 肝星状细胞 基因表达 

分 类 号：R575.2[医药卫生—消化系统]