Analysis of chloramphenicol in honey by on-line pretreatment liquid chromatography-tandem mass spectrometry  

作　　者：Shin-ichi Kawano Hong-Yuan Hao Yuki Hashi Jin-Ming Lin 

机构地区：[1]Department of Chemistry,Beijing Key Laboratory of Microanalytical Methods and Instrumentation,Tsinghua University [2]Shimadzu Global COE for Application & Technical Development,Shimadzu(China)Co.,Ltd.

出　　处：《Chinese Chemical Letters》2015年第1期36-38,共3页中国化学快报（英文版）

基　　金：supported by the National Natural Science Foundation of China(No.21227006);the Research Fund for the Doctoral Program of Higher Education(No.20110002110052)

摘　　要：hloramphenicol is an antibiotic and one of the potential contaminants in honey.Solid-phase extraction is the key pretreatment procedure for analysis of chloramphenicol in honey.In this work,an on-line pretreatment liquid chromatography-tandem mass spectrometer system for sensitive,reliable and higher throughput analysis was developed.With the methylcellulose-immobilized reversed-phase column,sugars in a honey sample were efficiently removed in 1 min.As a result,the limit of quantitation of chloramphenicol was 20 pg/mL（0.2 μg/kg honey）.hloramphenicol is an antibiotic and one of the potential contaminants in honey.Solid-phase extraction is the key pretreatment procedure for analysis of chloramphenicol in honey.In this work,an on-line pretreatment liquid chromatography-tandem mass spectrometer system for sensitive,reliable and higher throughput analysis was developed.With the methylcellulose-immobilized reversed-phase column,sugars in a honey sample were efficiently removed in 1 min.As a result,the limit of quantitation of chloramphenicol was 20 pg/mL（0.2 μg/kg honey）.

关 键 词：On-line pretreatment Liquid chromatography-mass spectrometry CHLORAMPHENICOL HONEY 

分 类 号：TS207.3[轻工技术与工程—食品科学] O657.63[轻工技术与工程—食品科学与工程]